Omori Koichi, Nakamura Tatsuo, Kanemaru Shinichi, Kojima Hisayoshi, Magrufov Akhmar, Hiratsuka Yasuyuki, Shimizu Yasuhiko
Department of Otolaryngology, Fukushima Medical School, School of Medicine, Fukushima City (Omori), Japan.
Ann Otol Rhinol Laryngol. 2004 Aug;113(8):623-7. doi: 10.1177/000348940411300805.
The purpose of the present study was to evaluate the efficacy of cricoid regeneration via in situ tissue engineering in a canine larynx for the treatment of subglottic stenosis. As the tissue scaffold, a Marlex mesh tube coated by collagen sponge was used for a rigid airway framework and for tissue regrowth around the tube. On 5 dogs, the larynx was exposed and the anterior third of the cricoid cartilage was resected. The tube was anastomosed to the lower edge of the thyroid cartilage and to the first tracheal cartilage. By postoperative endoscopic examination at 3 to 7 months, no airway obstruction was observed in any of the dogs. There was granulation tissue in 2 dogs and slight mesh exposure in 1 dog, but they were asymptomatic. Confluent regeneration of the epithelium over the scaffold and good incorporation of the scaffold mesh into the host tissue were observed after surgery.
本研究的目的是评估通过原位组织工程在犬类喉部进行环状软骨再生以治疗声门下狭窄的疗效。作为组织支架，使用涂有胶原海绵的Marlex网片管作为刚性气道框架以及管周围组织再生的支架。对5只犬，暴露喉部并切除环状软骨的前三分之一。将该管吻合至甲状软骨下缘和第一气管软骨。术后3至7个月通过内镜检查，未观察到任何一只犬出现气道阻塞。2只犬有肉芽组织，1只犬有轻微网片暴露，但均无症状。术后观察到支架上皮的融合再生以及支架网片与宿主组织的良好整合。
