Omori Koichi, Tada Yasuhiro, Suzuki Teruhisa, Nomoto Yukio, Matsuzuka Takashi, Kobayashi Ken, Nakamura Tatsuo, Kanemaru Shinichi, Yamashita Masaru, Asato Ryo
Department of Otolaryngology, Fukushima Medical University, School of Medicine, Fukushima City, Japan.
Ann Otol Rhinol Laryngol. 2008 Sep;117(9):673-8. doi: 10.1177/000348940811700908.
The objective of the present study was to demonstrate the efficacy of the clinical application of in situ tissue engineering using a scaffolding technique for laryngeal and tracheal tissue.
We have developed a tissue scaffold made from a Marlex mesh tube covered by collagen sponge. Based on successful animal experimental studies, in situ tissue engineering with a scaffold implant was applied to repair the larynx and trachea in 4 patients.
In 1 patient with subglottic stenosis, the thyroid cartilage, cricoid cartilage, and cervical trachea with scarring and granulation were resected and reconstructed by use of the scaffold. In 3 patients with thyroid cancer, the trachea and cricoid cartilage with tumor invasion were resected and the scaffold was implanted into the defect. Postoperative endoscopy during the observation period of 8 to 34 months showed a well-epithelialized airway lumen without any obstruction.
Our current technique of in situ tissue engineering using a scaffold shows great potential for use in the regeneration of airway defects.
本研究的目的是证明使用支架技术进行原位组织工程在喉和气管组织临床应用中的疗效。
我们研发了一种由覆盖胶原海绵的Marlex网片管制成的组织支架。基于成功的动物实验研究，将带支架植入物的原位组织工程应用于4例患者的喉和气管修复。
1例声门下狭窄患者，切除有瘢痕和肉芽组织的甲状软骨、环状软骨及颈段气管，并用支架进行重建。3例甲状腺癌患者，切除受肿瘤侵犯的气管和环状软骨，将支架植入缺损处。在8至34个月的观察期内，术后内镜检查显示气道腔上皮化良好，无任何梗阻。
我们目前使用支架的原位组织工程技术在气道缺损再生方面显示出巨大的应用潜力。
