Boskabady M H, Ramazani-Assari M
Department of Physiology, Ghaem Medical Centre, Mashhad University of Medical Sciences, 91735, Mashhad, Iran.
J Ethnopharmacol. 2001 Jan;74(1):83-8. doi: 10.1016/s0378-8741(00)00314-7.
We have studied the relaxant effect of Pimpinella anisum on isolated guinea pig tracheal chains and its possible mechanism(s). The bronchodilatory effects of aqueous and ethanol extracts and essential oil were examined on precontracted isolated tracheal chains of the guinea pig by 10 microM methacholine in two different conditions including: non-incubated tissues (group 1) and incubated tissues with 1 microM propranolol and 1 microM chlorpheniramine (group 2). In addition, the anticholinergic effects of essential oil and 10 nM atropine were tested by comparing the cumulative log concentration-response curves of methacholine induced contraction of tracheal chains and the effective concentration of methacholine, causing 50% of maximum response (EC(50)) in the presence of essential oil or atropine. Aqueous and ethanol extracts, essential oil and theophylline (1 mM) showed significant relaxant effects compared to those of controls. Although relaxant effect of essential oil was lower than theophylline, there was no significant difference between the effect of aqueous and ethanol extracts and that of theophylline. There was also no significant difference between the relaxant effects obtained in group 1 and 2 experiments. The results also showed parallel rightward shifts of methacholine-response curves and significant increase in EC(50) with the presence of atropine or essential oil. These results indicated bronchodilatory effects of essential oil, aqueous, and ethanol extracts from P. anisum. The results also showed that the relaxant effect of this plant is not due to an inhibitory effect of histamine (H(1)) or stimulatory effect of beta(2)-adrenergic receptors, but due to inhibitory effects on muscarinic receptors.
我们研究了茴芹对豚鼠离体气管链的舒张作用及其可能的机制。通过在两种不同条件下用10微摩尔甲胆碱对豚鼠离体气管链进行预收缩，检测了水提取物、乙醇提取物和精油的支气管舒张作用，这两种条件分别为：未孵育组织（第1组）和用1微摩尔普萘洛尔和1微摩尔氯苯那敏孵育的组织（第2组）。此外，通过比较甲胆碱诱导气管链收缩的累积对数浓度-反应曲线以及在精油或阿托品存在下引起最大反应50%的甲胆碱有效浓度（EC50），测试了精油和10纳摩尔阿托品的抗胆碱能作用。与对照组相比，水提取物、乙醇提取物、精油和茶碱（1毫摩尔）均显示出显著的舒张作用。尽管精油的舒张作用低于茶碱，但水提取物和乙醇提取物的作用与茶碱之间没有显著差异。在第1组和第2组实验中获得的舒张作用之间也没有显著差异。结果还表明，在阿托品或精油存在下，甲胆碱反应曲线平行右移，EC50显著增加。这些结果表明茴芹精油、水提取物和乙醇提取物具有支气管舒张作用。结果还表明，该植物的舒张作用不是由于组胺（H1）的抑制作用或β2-肾上腺素能受体的刺激作用，而是由于对毒蕈碱受体的抑制作用。
