Survival of and on Lettuce after Irrigation with Contaminated Water in a Temperate Climate.

Microbial disease outbreaks related to fresh produce consumption, including leafy green vegetables, have increased in recent years. Where contamination occurs, pathogen persistence may represent a risk for consumers' health. This study analysed the survival of and on lettuce plants watered with contaminated irrigation water via a single irrigation event and within stored irrigation water. Separate lettuce plants ( var. ) were irrigated with water spiked with Log 7 cfu/mL of each of the two strains and survival assessed via direct enumeration, enrichment and qPCR. In parallel, individual 20 L water microcosms were spiked with Log 7 cfu/mL of the individual strains and sampled at similar time points. Both strains were observed to survive on lettuce plants up to 28 days after inoculation. Direct quantification by culture methods showed a Log 4 decrease in the concentration of 14 days after inoculation, and a Log 3 decrease in the concentration of 10 days after inoculation. was detected in water samples up to 7 days after inoculation and was detected up to 28 days by direct enumeration. Both strains were recovered from enriched samples up to 28 days after inoculation. These results demonstrate that and strains are able to persist on lettuce after a single contamination event up until the plants reach a harvestable state. Furthermore, the persistence of and in water for up to 28 days after inoculation illustrates the potential for multiple plant contamination events from stored irrigation water, emphasising the importance of ensuring that irrigation water is of a high quality.