Keramas Georgios, Bang Dang Duong, Lund Marianne, Madsen Mogens, Bunkenborg Henrik, Telleman Pieter, Christensen Claus Bo Vöge
MIC-Department of Micro and Nanotechnology, Technical University of Denmark, DTU-Building 345 east, DK-2800 Kongens Lyngby, Denmark.
J Clin Microbiol. 2004 Sep;42(9):3985-91. doi: 10.1128/JCM.42.9.3985-3991.2004.
A DNA microarray for detection of Campylobacter spp. was recently developed and applied to detect Campylobacter spp. directly from chicken feces. Sixty-five pooled chicken cloacal swab samples from 650 individual broiler chickens were included in the study. The results of Campylobacter sp. detection obtained with DNA microarrays were compared to those obtained by conventional culture and gel electrophoresis. By conventional culture, 60% of the samples were positive for either Campylobacter jejuni or Campylobacter coli. By PCR and capillary electrophoresis, 95% of the samples were positive for Campylobacter spp., whereas with DNA microarrays all samples were positive for Campylobacter spp. By application of DNA microarray analysis, the isolates in 4 samples (6%) could not be identified to the species level, whereas by PCR-capillary electrophoresis, the isolates in 12 samples (19%) remained unidentified. Interestingly, PCR-capillary electrophoresis analysis revealed that two (3%) of the samples were positive for both C. jejuni and C. coli, while DNA microarray analysis revealed that nine (14%) of the samples were positive for both species. Of 65 samples, 2 samples were identified to contain C. coli by conventional culture but were positive for C. jejuni by both PCR-capillary electrophoresis and DNA microarray analysis. The discrepancy between the methods is discussed.
一种用于检测弯曲杆菌属的DNA微阵列最近被开发出来，并应用于直接从鸡粪便中检测弯曲杆菌属。该研究纳入了来自650只个体肉鸡的65份合并鸡泄殖腔拭子样本。将DNA微阵列检测弯曲杆菌属的结果与传统培养和凝胶电泳获得的结果进行了比较。通过传统培养，60%的样本对空肠弯曲杆菌或结肠弯曲杆菌呈阳性。通过聚合酶链反应（PCR）和毛细管电泳，95%的样本对弯曲杆菌属呈阳性，而使用DNA微阵列时所有样本对弯曲杆菌属均呈阳性。通过应用DNA微阵列分析，4个样本（6%）中的分离株无法鉴定到种水平，而通过PCR-毛细管电泳，12个样本（19%）中的分离株仍无法鉴定。有趣的是，PCR-毛细管电泳分析显示，两个（3%）样本对空肠弯曲杆菌和结肠弯曲杆菌均呈阳性，而DNA微阵列分析显示，九个（14%）样本对这两个种均呈阳性。在65个样本中，有2个样本通过传统培养鉴定为含有结肠弯曲杆菌，但通过PCR-毛细管电泳和DNA微阵列分析对空肠弯曲杆菌呈阳性。讨论了这些方法之间的差异。
