Chuma T, Hashimoto S, Okamoto K
Department of Veterinary Public Health, Faculty of Agriculture, Kagoshima University, Japan.
J Vet Med Sci. 2000 Dec;62(12):1291-5. doi: 10.1292/jvms.62.1291.
The best combination of primers and the annealing temperature of multiplex PCR for Campylobacter jejuni, Campylobacter coli, and Campylobacter lari were examined. The multiplex PCR was able to detect type strains of the three species. All results of identification of wild strains (30 strains of C. jejuni, 20 strains of C. coli, and 4 strains of C. lari) by the multiplex PCR coincided with those of the conventional biochemical identification tests, suggesting that the multiplex PCR can simultaneously differentiate C. jejuni, C. coli, and C. lari from wild strains of campylobacters easily and rapidly. Campylobacters were detected from sparrow feces by the multiplex PCR and antimicrobial sensitivities of the strains were determined to discuss the role of sparrows in contamination of broilers with C. jejuni. Three out of 13 strains of C. jejuni isolated from sparrow feces showed quinolone resistance. From the frequent use of quinolones for treatment of industrial animals like chickens, pigs, and cows, the three strains of quinolone-resistant C. jejuni in sparrows must have been originated from those industrial animals. Sparrows that have quinolone-resistant C. jejuni were considered to have contacted with industrial animals or thier feed. It may be presumed, on the contrary, that C. jejuni in sparrows could be a potential source of contamination of broilers.
研究了用于空肠弯曲菌、结肠弯曲菌和海鸥弯曲菌多重PCR的最佳引物组合及退火温度。该多重PCR能够检测这三种菌的模式菌株。通过多重PCR对野生菌株（30株空肠弯曲菌、20株结肠弯曲菌和4株海鸥弯曲菌）进行鉴定的所有结果与传统生化鉴定试验的结果一致，这表明多重PCR能够轻松、快速地同时从弯曲菌野生菌株中区分空肠弯曲菌、结肠弯曲菌和海鸥弯曲菌。通过多重PCR从麻雀粪便中检测到弯曲菌，并测定了菌株的抗菌敏感性，以探讨麻雀在空肠弯曲菌污染肉鸡中的作用。从麻雀粪便中分离出的13株空肠弯曲菌中有3株对喹诺酮耐药。由于鸡、猪和牛等养殖动物频繁使用喹诺酮进行治疗，麻雀体内的这3株喹诺酮耐药空肠弯曲菌必定源自这些养殖动物。携带喹诺酮耐药空肠弯曲菌的麻雀被认为与养殖动物或其饲料有过接触。相反，可以推测麻雀体内的空肠弯曲菌可能是肉鸡污染的一个潜在来源。
