de Kok J B, d'Oliveira C, Jongejan F
Department of Parasitology and Tropical Veterinary Medicine, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Exp Appl Acarol. 1993 Nov;17(11):839-46. doi: 10.1007/BF00225857.
Adult Hyalomma ticks were examined for the presence of Theileria annulata infection using the Polymerase Chain Reaction (PCR). A 372 bp DNA fragment derived from the small ribosomal RNA gene of T. annulata was amplified from 45 out of 50 (90%) H. dromedarii ticks and from 36 out of 50 (72%) H. marginatum marginatum ticks. No product was amplified from non-infected control ticks. Restriction enzyme digestion with Sac II confirmed that the product was derived from the targeted T. annulata gene. As a further confirmation it was shown that both species of Hyalomma ticks were able to transmit T. annulata to experimental calves. PCR detection of Theileria parasites in ticks was compared with conventional staining of dissected salivary glands using methyl green pyronin and its comparative advantages are discussed.
使用聚合酶链反应（PCR）检测成年璃眼蜱是否感染环形泰勒虫。从50只单峰驼璃眼蜱中的45只（90%）以及50只边缘璃眼蜱中的36只（72%）扩增出了一段源自环形泰勒虫小核糖体RNA基因的372 bp DNA片段。未从未感染的对照蜱中扩增出产物。用Sac II进行限制性酶切证实该产物源自目标环形泰勒虫基因。进一步证实表明，这两种璃眼蜱均能够将环形泰勒虫传播给实验小牛。将蜱中泰勒虫寄生虫的PCR检测与使用甲基绿派洛宁对解剖的唾液腺进行常规染色进行了比较，并讨论了其相对优势。
