Härfast B, Huddlestone J R, Casali P, Merigan T C, Oldstone M B
J Immunol. 1981 Nov;127(5):2146-50.
At different times of exposure, interferon (IFN) enhanced and suppressed pokeweed mitogen- (PWM) induced IgG synthesis by human peripheral blood lymphocytes (PBL). Pretreatment of PBL and IFN frequently increased antibody production by more than 100% when compared with that by untreated PBL. Results of experiments in which PBL were separated into T and B subpopulations indicated that IFN preparations acted directly on B cells. Thus, mixtures of IFN-treated B cells and untreated T cells from 5 of 7 persons tested produced 81% to 500% more IgG than untreated, matched control cells. However, IFN-treated monocytes mixed with untreated B and T cells or IFN-treated T cells mixed with untreated B cells failed to enhance IgG production significantly in similar assays. In contrast to the pretreatment protocol, when IFN was present in the incubation mixture throughout the PWM assay, IgG production decreased. Sephadex chromatography of the IFN and tests of the resulting fractions indicated that the IgG production-enhancing activity was located in the fraction carrying the antiviral activity.
在不同的暴露时间，干扰素（IFN）可增强和抑制人外周血淋巴细胞（PBL）对商陆丝裂原（PWM）诱导的IgG合成。与未处理的PBL相比，PBL和IFN的预处理常常使抗体产生增加超过100%。将PBL分离为T和B亚群的实验结果表明，IFN制剂直接作用于B细胞。因此，在7名测试者中，有5名的经IFN处理的B细胞与未处理的T细胞的混合物产生的IgG比未处理的匹配对照细胞多81%至500%。然而，在类似试验中，经IFN处理的单核细胞与未处理的B和T细胞混合，或经IFN处理的T细胞与未处理的B细胞混合，均未能显著增强IgG产生。与预处理方案相反，当在整个PWM试验的孵育混合物中都存在IFN时，IgG产生减少。对IFN进行葡聚糖凝胶层析并对所得组分进行测试表明，IgG产生增强活性位于具有抗病毒活性的组分中。
