肺炎克雷伯菌奥泽亚种对除草剂溴苯腈的代谢
Metabolism of the herbicide bromoxynil by Klebsiella pneumoniae subsp. ozaenae.
作者信息
McBride K E, Kenny J W, Stalker D M
出版信息
Appl Environ Microbiol. 1986 Aug;52(2):325-30. doi: 10.1128/aem.52.2.325-330.1986.
Abstract
Enrichment of soil samples for organisms able to utilize the herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) as a nitrogen source yielded bacterial isolates capable of rapidly metabolizing this compound. One isolate, identified as Klebsiella pneumoniae subsp. ozaenae, could completely convert 0.05% bromoxynil to 3,5-dibromo-4-hydroxybenzoic acid and use the liberated ammonia as a sole nitrogen source. Assays of cell extracts of this organism for the ability to produce ammonia from bromoxynil revealed the presence of a nitrilase (EC 3.5.51) activity. The enzyme could not utilize 3,5-dibromo-4-hydroxybenzamide as a substrate, and no 3,5-dibromo-4-hydroxybenzamide could be detected as a product of bromoxynil transformation. Comparison of related aromatic nitriles as substrates demonstrated that the Klebsiella enzyme is highly specific for bromoxynil.
摘要
从土壤样品中富集能够利用除草剂溴苯腈（3,5 - 二溴 - 4 - 羟基苯甲腈）作为氮源的微生物，得到了能够快速代谢该化合物的细菌分离株。其中一个分离株被鉴定为肺炎克雷伯菌奥泽亚种，它能将0.05%的溴苯腈完全转化为3,5 - 二溴 - 4 - 羟基苯甲酸，并将释放出的氨作为唯一氮源。对该微生物的细胞提取物进行从溴苯腈产生氨的能力检测，结果显示存在腈水解酶（EC 3.5.51）活性。该酶不能利用3,5 - 二溴 - 4 - 羟基苯甲酰胺作为底物，并且在溴苯腈转化产物中未检测到3,5 - 二溴 - 4 - 羟基苯甲酰胺。以相关芳香腈作为底物进行比较表明，克雷伯菌中的这种酶对溴苯腈具有高度特异性。
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