Hill K, Boone C, Goebl M, Puccia R, Sdicu A M, Bussey H
Department of Biology, McGill University, Montreal, Quebec, Canada.
Genetics. 1992 Feb;130(2):273-83. doi: 10.1093/genetics/130.2.273.
We have cloned, sequenced and disrupted the KRE2 gene of Saccharomyces cerevisiae, identified by killer-resistant mutants with a defective cell wall receptor for the toxin. The KRE2 gene is close to PHO8 on chromosome 4, and encodes a predicted 49-kD protein, Kre2p, that probably enters the secretory pathway. Haploid cells carrying a disruption of the KRE2 locus grow more slowly than wild-type cells at 30 degrees, and fail to grow at 37 degrees. At 30 degrees, kre2 mutants showed altered N-linked glycosylation of proteins, as the average size of N-linked outer chains was reduced. We identified two other genes, YUR1 on chromosome 10, and KTR1 on chromosome 15, whose predicted products share 36% identity with Kre2p over more than 300 amino acid residues. Yur1p has an N-terminal signal sequence like Kre2p, while Ktr1p has a predicted topology consistent with a type 2 membrane protein. In all cases the conserved regions of these proteins appear to be on the lumenal side of secretory compartments, suggesting related function. KRE2, KTR1 and YUR1 define a new yeast gene family.
我们已经克隆、测序并破坏了酿酒酵母的KRE2基因，该基因是通过对毒素的细胞壁受体有缺陷的抗杀手突变体鉴定出来的。KRE2基因位于4号染色体上靠近PHO8的位置，编码一种预测分子量为49-kD的蛋白质Kre2p，它可能进入分泌途径。携带KRE2基因座破坏的单倍体细胞在30℃下比野生型细胞生长得更慢，在37℃下无法生长。在30℃时，kre2突变体显示蛋白质的N-连接糖基化发生改变，因为N-连接外链的平均大小减小。我们鉴定出另外两个基因，位于10号染色体上的YUR1和位于15号染色体上的KTR1，它们的预测产物在超过300个氨基酸残基上与Kre2p有36%的同一性。Yur1p具有与Kre2p类似的N端信号序列，而Ktr1p的预测拓扑结构与2型膜蛋白一致。在所有情况下，这些蛋白质的保守区域似乎位于分泌区室的腔侧，表明它们具有相关功能。KRE2、KTR1和YUR1定义了一个新的酵母基因家族。
