炎症性肠病中结肠固有层单个核细胞可溶性白细胞介素2受体的体外释放增加。
Increased in vitro release of soluble interleukin 2 receptor by colonic lamina propria mononuclear cells in inflammatory bowel disease.
作者信息
Schreiber S, Raedler A, Conn A R, Rombeau J L, MacDermott R P
机构信息
University of Pennsylvania, Gastrointestinal Section, Philadelphia, PA.
出版信息
Gut. 1992 Feb;33(2):236-41. doi: 10.1136/gut.33.2.236.
Increased concentrations of the soluble form of the interleukin 2 receptor have been observed in the sera of Crohn's disease and ulcerative colitis patients. In this study we have observed the spontaneous release of soluble interleukin 2 receptor by unstimulated, isolated normal and inflammatory bowel disease colonic lamina propria mononuclear cells. Lamina propria mononuclear cells from Crohn's disease patients (median = 204 U/ml (interquartile range 126-396, n 17) secreted significantly (p less than 0.01) more soluble interleukin 2 receptor than normal controls (median = 124.5 U/ml (108-131), n 12). No statistically significant differences were seen between ulcerative colitis (median = 135 U/ml (92-196), n 20) and normal controls. Moreover, significantly (p less than 0.01) increased amounts of soluble interleukin 2 receptor were secreted by colonic diverticulitis lamina propria mononuclear cells (median = 259 U/ml (149-282), n 15) which were used as disease specificity controls. Time course experiments showed that the majority of soluble interleukin 2 receptor was released by isolated lamina propria mononuclear cells in the first six days of culture. Upon stimulation with pokeweed mitogen, Crohn's disease (median = 2258 U/ml (1435-3584), n 14), normal control (median = 2622 U/ml (2030-3180), n 14) and diverticulitis lamina propria mononuclear cells (median = 2745 U/ml (1733-3192), n 10) reached similar maximal soluble interleukin 2 receptor secretion levels, while ulcerative colitis lamina propria mononuclear cells secreted significantly (p less than 0.005) less soluble interleukin 2 receptor (median = 912 U/ml (494-1259), n 17). These results suggest that enhanced shedding/secretion of soluble interleukin 2 receptor by intestinal lymphocytes may account in part for increased serum soluble interleukin 2 receptor concentrations during chronic intestinal inflammatory reactions.
在克罗恩病和溃疡性结肠炎患者的血清中，已观察到白细胞介素2受体可溶性形式的浓度升高。在本研究中，我们观察了未受刺激的、分离出的正常和炎性肠病结肠固有层单核细胞自发释放可溶性白细胞介素2受体的情况。来自克罗恩病患者的固有层单核细胞（中位数 = 204 U/ml（四分位间距126 - 396，n = 17））分泌的可溶性白细胞介素2受体明显（p < 0.01）多于正常对照组（中位数 = 124.5 U/ml（108 - 131），n = 12）。溃疡性结肠炎患者（中位数 = 135 U/ml（92 - 196），n = 20）与正常对照组之间未观察到统计学上的显著差异。此外，用作疾病特异性对照的结肠憩室炎固有层单核细胞（中位数 = 259 U/ml（149 - 282），n = 15）分泌的可溶性白细胞介素2受体量显著（p < 0.01）增加。时间进程实验表明，大多数可溶性白细胞介素2受体是由分离出的固有层单核细胞在培养的前六天释放的。在用商陆有丝分裂原刺激后，克罗恩病（中位数 = 2258 U/ml（1435 - 3584），n = 14）、正常对照（中位数 = 2622 U/ml（2030 - 3180），n = 14）和憩室炎固有层单核细胞（中位数 = 2745 U/ml（1733 - 3192），n = 10）达到相似的最大可溶性白细胞介素2受体分泌水平，而溃疡性结肠炎固有层单核细胞分泌的可溶性白细胞介素2受体明显（p < 0.005）较少（中位数 = 912 U/ml（494 - 1259），n = 17）。这些结果表明，肠道淋巴细胞增强可溶性白细胞介素2受体的脱落/分泌可能部分解释了慢性肠道炎症反应期间血清可溶性白细胞介素2受体浓度的升高。
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