Purvén M, Lagergård T
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
Infect Immun. 1992 Mar;60(3):1156-62. doi: 10.1128/iai.60.3.1156-1162.1992.
An extra- and an intracellular product from the bacterium Haemophilus ducreyi were shown to have a cytotoxic effect on cell lines of epithelial origin, e.g., HEp-2 and HeLa cells. The cytotoxic effect appeared on cells within 24 h and resulted in cell death and morphologically changed cells. The cytotoxic activity was heat and pronase sensitive. The activity could be removed by incubation with the target cells, suggesting attachment of the agent to the cells. The cytotoxic products were secreted into the environment during exponential growth of bacteria and produced by most of the H. ducreyi strains tested. The activity was neutralized by homologous rabbit immune serum but not by the corresponding preimmune serum. The results indicate that strains of H. ducreyi produce a cytotoxin which may be of importance to the pathogenesis of chancroid.
研究表明，杜克雷嗜血杆菌的一种胞外产物和一种胞内产物对上皮来源的细胞系（如HEp-2和HeLa细胞）具有细胞毒性作用。细胞毒性作用在24小时内出现在细胞上，导致细胞死亡和细胞形态改变。细胞毒性活性对热和链霉蛋白酶敏感。该活性可通过与靶细胞孵育而去除，表明该因子附着于细胞。细胞毒性产物在细菌指数生长期间分泌到环境中，并且在所测试的大多数杜克雷嗜血杆菌菌株中产生。该活性被同源兔免疫血清中和，但不被相应的免疫前血清中和。结果表明，杜克雷嗜血杆菌菌株产生一种细胞毒素，这可能对软下疳的发病机制具有重要意义。
