Beno D W, Mathews H L
Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University of Chicago, Maywood, Illinois 60153.
Infect Immun. 1992 Mar;60(3):853-63. doi: 10.1128/iai.60.3.853-863.1992.
Murine splenocytes, Percoll-enriched low-density lymphocytes, and interleukin-2 (IL-2)-activated lymphocytes were assessed for the capacity to limit the growth of the hyphal form of Candida albicans. No fungal-growth-inhibitory activity was exhibited for C. albicans by either splenocytes or Percoll-enriched lymphocytes. These cells were capable of cytotoxic activity for a natural killer cell-sensitive cell line. However, when cultured for several days with IL-2, splenocytes acquired the capacity to inhibit the growth of the fungus. The appearance of the antifungal activity coincided with the development of cytotoxic activity for the natural killer cell-insensitive cell line. Anti-C. albicans and antitumor activities of IL-2-activated lymphocytes were competitively and reciprocally inhibited by C. albicans and the natural killer cell-sensitive and -insensitive cell lines. The antifungal activity of the IL-2-activated lymphocytes was exhibited against a number of clinical isolates of C. albicans and related fungal species. IL-2-activated human peripheral blood lymphocytes also acquired the capacity to inhibit the growth of C. albicans. These data show that in vitro growth inhibition can be mediated by IL-2-stimulated lymphocytes which are neither fungal strain nor mammalian species restricted in their biological activity.
对小鼠脾细胞、经Percoll富集的低密度淋巴细胞以及白细胞介素-2（IL-2）激活的淋巴细胞限制白色念珠菌菌丝体生长的能力进行了评估。脾细胞或经Percoll富集的淋巴细胞对白色念珠菌均未表现出真菌生长抑制活性。这些细胞对自然杀伤细胞敏感的细胞系具有细胞毒性活性。然而，当脾细胞与IL-2一起培养数天时，它们获得了抑制真菌生长的能力。抗真菌活性的出现与对自然杀伤细胞不敏感的细胞系细胞毒性活性的发展相一致。白色念珠菌以及自然杀伤细胞敏感和不敏感的细胞系竞争性地、相互抑制IL-2激活的淋巴细胞的抗白色念珠菌和抗肿瘤活性。IL-2激活的淋巴细胞的抗真菌活性针对多种白色念珠菌临床分离株及相关真菌物种表现出来。IL-2激活的人外周血淋巴细胞也获得了抑制白色念珠菌生长的能力。这些数据表明，体外生长抑制可由IL-2刺激的淋巴细胞介导，其生物活性不受真菌菌株或哺乳动物物种的限制。
