Beno D W, Mathews H L
Department of Microbiology, Stritch School of Medicine, Loyola University of Chicago, Maywood, Illinois 60153.
Cell Immunol. 1990 Jun;128(1):89-100. doi: 10.1016/0008-8749(90)90009-g.
Previous reports have demonstrated natural killer cells (NK) to exert growth inhibitory effects against certain fungi, but not against Candida albicans. In this investigation, interleukin-2 (IL-2)-induced lymph node cells with phenotypic and functional characteristics of NK were shown to inhibit the growth of C. albicans. Growth inhibition was evaluated by both the release of 51Cr by the fungus and the inhibition of microcolony growth of the fungus on Sabouraud's dextrose agar. Lymphoid cells derived from C57Bl/6 mice and immediately assessed for hyphal growth inhibition showed little or no activity. However, significant hyphal growth inhibition was produced by lymph node cells cultured with recombinant IL-2. Growth inhibitory activity was dependent upon the concentration of IL-2 and was mediated by nonadherent lymphocytes which lysed an NK-susceptible and to a lesser extent an NK-resistant cell line. Treatment of the IL-2-induced cells with anti-asialo GM1 but not anti-Thy-1 and complement abrogated growth inhibition of C. albicans. These results suggest that IL-2-induced lymph node cells with functional and phenotypic characteristics similar to those of activated NK, mediate in vitro growth inhibition of the hyphal form of C. albicans.
先前的报告表明，自然杀伤细胞（NK）对某些真菌具有生长抑制作用，但对白色念珠菌没有作用。在本研究中，具有NK表型和功能特征的白细胞介素-2（IL-2）诱导的淋巴结细胞显示出抑制白色念珠菌生长的作用。通过真菌释放51Cr以及抑制真菌在沙氏葡萄糖琼脂上的微菌落生长来评估生长抑制情况。来自C57Bl/6小鼠的淋巴细胞，立即评估其对菌丝生长的抑制作用，几乎没有或没有活性。然而，用重组IL-2培养的淋巴结细胞产生了显著的菌丝生长抑制作用。生长抑制活性取决于IL-2的浓度，并且由非贴壁淋巴细胞介导，这些细胞能裂解对NK敏感以及在较小程度上对NK有抗性的细胞系。用抗唾液酸GM1而非抗Thy-1和补体处理IL-2诱导的细胞，可消除对白色念珠菌的生长抑制作用。这些结果表明，具有与活化NK相似功能和表型特征的IL-2诱导的淋巴结细胞，在体外介导白色念珠菌菌丝体形式的生长抑制作用。
