Velde J T, Burkhardt R, Kleiverda K, Leenheers-Binnendijk L, Sommerfeld W
Histopathology. 1977 Sep;1(5):319-30. doi: 10.1111/j.1365-2559.1977.tb01671.x.
Methyl-methacrylate embedding makes it possible to obtain semi-thin sections rich in detail and without tissue shrinkage. The procedure requires considerable time and labour, and great care must be taken to prevent heat damage to the tissue during the exothermic polymerization process. The original method given by Burkhardt (1966) and later modifications are described and discussed, with special attention to the practical problems encountered and their solutions.
甲基丙烯酸甲酯包埋能够获得细节丰富且无组织收缩的半薄切片。该过程需要相当多的时间和人力，并且在放热聚合过程中必须格外小心以防止组织受热损伤。文中描述并讨论了伯克哈特（1966年）给出的原始方法及其后续改进，特别关注了所遇到的实际问题及其解决方案。
