针对混合热灭活大肠菌群抗原和脂多糖核心抗原的尿免疫球蛋白G抗体。
Urinary IgG antibody against mixed heat-killed coliform antigen and lipopolysaccharide core antigen.
作者信息
Gibb A P, Edmond D M
机构信息
Department of Medical Microbiology, University of Edinburgh Medical School.
出版信息
J Clin Pathol. 1992 Feb;45(2):161-4. doi: 10.1136/jcp.45.2.161.
AIMS
To determine whether antibody to lipopolysaccharide-core (LPS-core) antigen is an important component of the antibody, detected by mixed heat-killed coliform antigen, in urine from patients with suspected urinary tract infection.
METHODS
LPS-core antigen and mixed heat-killed coliform antigen were used in an enzyme linked immunosorbent assay (ELISA) to measure IgG antibody in midstream urine samples. Seventy two samples from students attending their general practitioner with symptoms suggestive of urinary tract infection, six samples from which a Gram positive organism was isolated, and 16 asymptomatic controls were tested. Plates coated with LPS-core antigen were also used to absorb out the antibody detected by the mixed heat-killed coliform antigen.
RESULTS
Antibody to either antigen was associated with a positive culture, but neither was a useful predictor of a positive culture. There was a significant correlation between the results of the two assays (r = 0.7633; p less than 0.001), and absorption with LPS-core antigen did reduce the level of antibody to the mixed heat-killed coliform antigen. Antibody to both preparations was found in patients with Gram positive urinary tract infection.
CONCLUSION
Antibody to LPS-core antigen forms a substantial part of the antibody detected by mixed heat-killed coliform ELISA. The antibodies detected by these assays are probably the result of non-specific leakage of antibody into the urine, rather than a specific immune response.
目的
确定抗脂多糖核心（LPS核心）抗原抗体是否是通过混合热灭活大肠菌群抗原检测到的抗体的重要组成部分，该抗体存在于疑似尿路感染患者的尿液中。
方法
采用LPS核心抗原和混合热灭活大肠菌群抗原，通过酶联免疫吸附测定（ELISA）法检测中段尿样本中的IgG抗体。对72例出现提示尿路感染症状的全科门诊学生的样本、6例分离出革兰氏阳性菌的样本以及16例无症状对照样本进行检测。还用包被有LPS核心抗原的酶标板吸附通过混合热灭活大肠菌群抗原检测到的抗体。
结果
针对任何一种抗原的抗体都与培养阳性相关，但两者均不是培养阳性的有效预测指标。两种检测结果之间存在显著相关性（r = 0.7633；p < 0.001），用LPS核心抗原吸附确实降低了针对混合热灭活大肠菌群抗原的抗体水平。在革兰氏阳性尿路感染患者中发现了针对两种制剂的抗体。
结论
抗LPS核心抗原抗体是通过混合热灭活大肠菌群ELISA检测到的抗体的重要组成部分。这些检测所检测到的抗体可能是抗体非特异性漏入尿液的结果，而非特异性免疫反应所致。
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