用显色琼脂检测氟康唑耐药酵母的简易方法。
Simple method for detecting fluconazole-resistant yeasts with chromogenic agar.
作者信息
Patterson T F, Revankar S G, Kirkpatrick W R, Dib O, Fothergill A W, Redding S W, Sutton D A, Rinaldi M G
机构信息
Department of Medicine, University of Texas Health Science Center at San Antonio 78284, USA.
出版信息
J Clin Microbiol. 1996 Jul;34(7):1794-7. doi: 10.1128/JCM.34.7.1794-1797.1996.
Abstract
A method for detecting fluconazole-resistant yeasts was developed that uses chromogenic agar containing fluconazole. Yeasts were plated on media with fluconazole at 0, 8, and 16 micrograms/ml. On media without fluconazole, normal growth of susceptible yeasts (defined as those having a fluconazole MIC of < 8 micrograms/ml) was detected, while fluconazole-containing media suppressed susceptible strains and normal colonies of resistant yeasts (fluconazole MICs of > or = 8 micrograms/ml) were detected. This method was used to screen for resistance in oropharyngeal candidiasis. Isolates having fluconazole MICs of > or = 8 micrograms/ml and < 8 micrograms/ml were correctly predicted in 43 of 45 cultures and 115 of 116 cultures, respectively. This screening method appears to be rapid and sensitive for detection of fluconazole-resistant yeasts.
摘要
开发了一种检测氟康唑耐药酵母的方法，该方法使用含氟康唑的显色琼脂。将酵母接种在含0、8和16微克/毫升氟康唑的培养基上。在不含氟康唑的培养基上，可检测到敏感酵母（定义为氟康唑最低抑菌浓度<8微克/毫升的酵母）的正常生长，而含氟康唑的培养基可抑制敏感菌株，并检测到耐药酵母（氟康唑最低抑菌浓度≥8微克/毫升）的正常菌落。该方法用于筛查口咽念珠菌病中的耐药性。氟康唑最低抑菌浓度≥8微克/毫升和<8微克/毫升的分离株在45份培养物中的43份和116份培养物中的115份中分别被正确预测。这种筛查方法似乎对检测氟康唑耐药酵母快速且灵敏。
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