Hasanzadeh Keshteli Samaneh, Farsi Mir Mehrdad, Khafri Soraya
Department of Anatomical Sciences, School of Medicine, Babol University of Medical Sciences, Babol, Iran.
Department of Biostatistics and Epidemiology, Babol University of Medical Sciences, Babol, Iran.
Int J Mol Cell Med. 2016 Fall;5(4):246-254. Epub 2016 Nov 13.
Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-15%, >15% for normal morphology; <50%, 50-70%, >70 % for motility; and <10, 10-20, 21-34, 35-50, >50 million/ml for concentration. SDF analysis was performed and values under 30% were assumed to be normal. Normal range of HOST was considered to be >60%.Only normal sperm morphology had significant relationship with DF rate (P<0.001). Normal morphology, motility, and concentration of sperms had significant relationship with HOST (P<0.001, 0.05, and <0.003,respectively). There was a significant negative correlation between sperm morphology and DF rate. The correlations between sperm parameters and percentage of HOST were significantly positive (r: 0.44, 0.19, and 0.32 for morphology, motility, and concentration, respectively). Receiver operating characteristic curve (ROC) showed that sperm morphology is a strong predictor of the rate of DF and HOST (accuracy: 0.74' and 0.81, respectively). The best sperm morphology cut off point for DF and HOST rate prediction was 4.5% and 5.5%, respectively.Sperm morphology had significant correlation with DF rate and HOST and is supposed to be a predictor for these tests. Performing these three tests collectively for evaluation of semen samples would not be necessarily required in all cases.
精液分析、精子DNA碎片率（SDF）和低渗肿胀试验（HOST）通常用于评估精子的受精能力。在男性不育症检查中，是否纳入SDF和HOST存在一些争议。通过三次精液分析，对77名男性的精液进行了SDF和HOST评估。精子参数分为不同类别：正常形态分别为<5%、5-15%、>15%；活力分别为<50%、50-70%、>70%；浓度分别为<10、10-20、21-34、35-50、>5000万/ml。进行了SDF分析，30%以下的值被认为是正常的。HOST的正常范围被认为是>60%。只有正常精子形态与DF率有显著关系（P<0.001）。精子的正常形态、活力和浓度与HOST有显著关系（分别为P<0.001、0.05和<0.003）。精子形态与DF率之间存在显著负相关。精子参数与HOST百分比之间的相关性显著为正（形态、活力和浓度的r分别为0.44、0.19和0.32）。受试者工作特征曲线（ROC）显示，精子形态是DF率和HOST的有力预测指标（准确率分别为0.74和0.81）。预测DF率和HOST率的最佳精子形态截断点分别为4.5%和5.5%。精子形态与DF率和HOST显著相关，被认为是这些检测的预测指标。并非在所有情况下都需要同时进行这三项检测来评估精液样本。
