Griesbeck Oliver
Nachwuchsgruppe Zelluläre Dynamik, Max-Planck-Institut für Neurobiologie, Am Klopferspitz 18, 82152 Martinsried, Germany.
Curr Opin Neurobiol. 2004 Oct;14(5):636-41. doi: 10.1016/j.conb.2004.08.002.
The exploitation of green fluorescent protein-based biosensors promises to revolutionize functional imaging of the nervous system. Various approaches have created a multitude of reporters of neuronal activity and of activation of biochemical signaling pathways. Although the number of different probes has increased significantly, the critical step remains to bring these probes from the cuvette through the imaging of single cells to the imaging of whole organisms in vivo. The recent development of new genetically encoded sensors and their functional expression in model organisms are encouraging signs that the field is moving ahead in this direction.
基于绿色荧光蛋白的生物传感器的开发有望彻底改变神经系统的功能成像。各种方法已创造出众多神经元活动及生化信号通路激活的报告分子。尽管不同探针的数量显著增加，但关键步骤仍是将这些探针从比色皿通过单细胞成像推进到体内全生物体成像。新型基因编码传感器的最新进展及其在模式生物中的功能表达，是该领域正朝此方向前进的令人鼓舞的迹象。
