Stephanopoulos Gregory, Alper Hal, Moxley Joel
Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56-469, Cambridge, MA 02139, USA.
Nat Biotechnol. 2004 Oct;22(10):1261-7. doi: 10.1038/nbt1016.
Cellular complexity makes it difficult to build a complete understanding of cellular function but also offers innumerable possibilities for modifying the cellular machinery to achieve a specific purpose. The exploitation of cellular complexity for strain improvement has been a challenging goal for applied biological research because it requires the coordinated understanding of multiple cellular processes. It is therefore pursued most efficiently in the framework of systems biology. Progress in strain improvement will depend not only on advances in technologies for high-throughput measurements but, more importantly, on the development of theoretical methods that increase the information content of these measurements and, as such, facilitate the elucidation of mechanisms and the identification of genetic targets for modification.
细胞的复杂性使得全面理解细胞功能变得困难，但也为改造细胞机制以实现特定目的提供了无数可能性。利用细胞复杂性来改进菌株一直是应用生物学研究的一个具有挑战性的目标，因为这需要对多个细胞过程进行协同理解。因此，在系统生物学的框架内能够最有效地实现这一目标。菌株改良的进展不仅将取决于高通量测量技术的进步，更重要的是，取决于理论方法的发展，这些方法能够增加这些测量的信息含量，从而有助于阐明机制并识别用于改造的遗传靶点。
