Poolman M G, Venkatesh K V, Pidcock M K, Fell D A
School of Biology and Molecular Science Oxford Brookes University, Headington, Oxford OX3 OBP, United Kingdom.
Biotechnol Bioeng. 2004 Dec 5;88(5):601-12. doi: 10.1002/bit.20273.
In this article we address the question of how, given information about the reaction fluxes of a system, flux values can be assigned to the elementary modes of that system. Having described a method by which this may be accomplished, we first illustrate its application to a hypothetical, in silico system, and then apply it to fermentation data from Lactobacillus rhamnosus. This reveals substantial changes in the flux values assigned to elementary modes, and thus to the internal metabolism, as the fermentation progresses. This is information that could not, to our knowledge, be obtained by existing methods. The relationship between our technique and the well-known method of Metabolic Flux Analysis is also discussed.
在本文中，我们探讨了这样一个问题：在已知一个系统的反应通量信息的情况下，如何为该系统的基本模式分配通量值。在描述了一种实现此目的的方法后，我们首先说明其在一个虚拟的计算机系统中的应用，然后将其应用于来自鼠李糖乳杆菌的发酵数据。这揭示了随着发酵的进行，分配给基本模式的通量值以及由此导致的内部代谢发生了显著变化。据我们所知，现有方法无法获得这些信息。我们还讨论了我们的技术与著名的代谢通量分析方法之间的关系。
