Ytrehus Bjørnar, Andreas Haga H, Mellum Cato N, Mathisen Lene, Carlson Cathy S, Ekman Stina, Teige Jon, Reinholt Finn P
Division of Anatomy and Pathology, Department of Basal Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, PO Box 8146 Dep., N-0033 Oslo.
J Orthop Res. 2004 Nov;22(6):1201-9. doi: 10.1016/j.orthres.2004.03.006.
Osteochondrosis is a disorder of growth cartilage in which a focal failure of blood supply has been proposed as an important initiating factor. In the present study we investigated the effect on epiphyseal growth cartilage of experimentally interrupting the blood supply to a limited area of the distal femur of growing pigs. In 12 pigs, a thin full-thickness cartilage slab was removed from the abaxial margin of the medial condyle, thereby transecting a limited number of cartilage canals. The pigs were culled 1, 2, 3, 7, 14, 21 and 29 days post-surgery. The condylar cartilage was studied histologically, immunohistologically and by use of the TUNEL method. The transection induced cellular death of cartilage canal elements followed by cellular death of chondrocytes within the deep layers of the resting zone of the epiphyseal growth cartilage. However, in the superficial layers of the resting zone, chondrocytes appeared to proliferate into and subsequently chondrify some of the necrotic cartilage canals. The dying and dead cells were TUNEL-positive, but active caspase 3-negative. The loss of vascular supply induced increased VEGF-immunostaining in chondrocytes surrounding the affected area. We conclude that transection of cartilage canals produces chondronecrosis in the deep resting zone of the epiphyseal growth cartilage similar to that observed in spontaneously occurring osteochondrosis.
骨软骨病是一种生长软骨疾病，其中局部血液供应衰竭被认为是一个重要的起始因素。在本研究中，我们研究了实验性中断生长猪股骨远端有限区域的血液供应对骺板生长软骨的影响。在12头猪中，从内侧髁的背外侧边缘切除一块薄的全层软骨板，从而横断有限数量的软骨管。在术后1、2、3、7、14、21和29天对猪实施安乐死。对髁软骨进行组织学、免疫组织学研究，并使用TUNEL法进行检测。横断导致软骨管内细胞死亡，随后骺板生长软骨静止区深层的软骨细胞死亡。然而，在静止区的表层，软骨细胞似乎增殖进入一些坏死的软骨管并随后软骨化。死亡和濒死细胞TUNEL染色呈阳性，但活化的半胱天冬酶-3染色呈阴性。血管供应丧失导致受影响区域周围软骨细胞中VEGF免疫染色增加。我们得出结论，软骨管横断在骺板生长软骨的深层静止区产生软骨坏死，类似于在自然发生的骨软骨病中观察到的情况。
