Berriatua E, Barandika J, Aduriz G, Atxaerandio R, Garrido J, García-Pérez A L
Sanidad Animal, Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER), Berreaga 1, Derio, Bizkaia 48160, Spain.
Vet J. 2004 Nov;168(3):336-42. doi: 10.1016/j.tvjl.2003.11.005.
Using p125/p80 antibody and antigen-ELISA tests, age-specific seroprevalence and presence of persistently infected (PI) sheep were investigated in six commercial latxa dairy-flocks, housed for variable periods. The flocks all had a recent history of Border disease (BD). Every flock included seropositive sheep and seven 0.5-3-year-old PI sheep were detected in two of four flocks tested. Age-specific antibody patterns differed according to the presence or absence of PI sheep in the flock. In flocks free of PI sheep, seroprevalence was 6-13% in 1-year-old sheep and 42-93% in older sheep. In contrast, seroprevalence was 67-99% in sheep raised with PI sheep for at least 1 year and 29-33% in replacement 0.5-0.6-year-old sheep (including a PI sheep) indicating that Border disease virus (BDV) transmission in Basque dairy-flocks can be relatively slow. Moderate seroprevalence in young replacement sheep should not discourage further testing to detect PI sheep, and our results highlight the risk of failing to achieve "natural vaccination" prior to pregnancy by mixing PI sheep with BDV-unexposed ewes.
使用p125/p80抗体和抗原酶联免疫吸附测定（ELISA）试验，在六个商业化拉查乳羊群中调查了不同饲养期的特定年龄血清阳性率和持续感染（PI）绵羊的存在情况。这些羊群近期均有边界病（BD）病史。每个羊群都有血清阳性绵羊，并且在检测的四个羊群中的两个中检测到了7只0.5至3岁的PI绵羊。特定年龄的抗体模式因羊群中是否存在PI绵羊而有所不同。在没有PI绵羊的羊群中，1岁绵羊的血清阳性率为6%-13%，年龄较大的绵羊为42%-93%。相比之下，与PI绵羊一起饲养至少1年的绵羊血清阳性率为67%-99%，0.5至0.6岁的替代绵羊（包括一只PI绵羊）血清阳性率为29%-33%，这表明巴斯克乳羊群中边界病病毒（BDV）的传播可能相对缓慢。年轻替代绵羊中的中等血清阳性率不应阻碍进一步检测以发现PI绵羊，并且我们的结果突出了在怀孕前将PI绵羊与未接触BDV的母羊混养而无法实现“自然免疫”的风险。
