Yu Chun-Fai, Yu Peter Hoi-Fu, Chan Pui-Ling, Yan Qun, Wong Po-Keung
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hunghom, Kowloon, Hong Kong, China.
Toxicon. 2004 Nov;44(6):641-7. doi: 10.1016/j.toxicon.2004.07.021.
Out of eight dominant discrete bacterial colonies isolated and purified from the toxic marine puffer fishes collected in Hong Kong waters, two novel species of non-sporing, non-acid-fast and chemoorganotrophic bacteria capable of producing tetrodotoxin (TTX, a potent non-protein neurotoxin), as well as one previously reported and confirmed TTX-producing bacterium. They were identified as Microbacterium arabinogalactanolyticum, Serratia marcescens and Vibrio alginolyticus, respectively, all of which are widely distributed in soils, sewage or marine environments. Each bacterial isolate (500 ml broth medium cultured in darkness without aeration for 10 days at 25 degrees C) could produce an amount of toxicity, after extraction and purification, ranging from 78.3 to 105.3 mouse units (MU) in 500 ml of broth medium by mouse bioassay. The principal toxic component in the bacterial cultures was determined to be TTX by thin layer chromatography and mass spectrometry.
从在香港水域采集的有毒海河豚中分离并纯化出的八个主要离散细菌菌落中，有两种新的非芽孢、非抗酸且能产生河豚毒素（TTX，一种强效非蛋白质神经毒素）的化能有机营养细菌，以及一种先前已报道并确认能产生TTX的细菌。它们分别被鉴定为阿拉伯半乳聚糖分解微杆菌、粘质沙雷氏菌和解藻酸弧菌，所有这些细菌都广泛分布于土壤、污水或海洋环境中。每种细菌分离株（在25℃黑暗且不通气的条件下，于500毫升肉汤培养基中培养10天）经提取和纯化后，通过小鼠生物测定法，在500毫升肉汤培养基中可产生78.3至105.3小鼠单位（MU）的毒性。通过薄层色谱法和质谱法确定细菌培养物中的主要有毒成分是TTX。
