从暗纹东方鲀中分离到一株具有产河豚毒素能力的溶纤维弧菌及其特性研究。

Isolation of a Lysinibacillus fusiformis strain with tetrodotoxin-producing ability from puffer fish Fugu obscurus and the characterization of this strain.

机构信息

Department of Life Sciences, Binzhou University, 391 Huanghe Road 5, Binzhou 256600, China.

出版信息

Toxicon. 2010 Sep 15;56(4):640-3. doi: 10.1016/j.toxicon.2010.05.011. Epub 2010 Jun 1.

DOI:10.1016/j.toxicon.2010.05.011

PMID:20576513

Abstract

Six dominant strains were isolated from the livers of the puffer fish Fugu obscurus and were screened for their TTX production-ability. Electrospray ionization-mass spectrometry (ESI-MS) analyses revealed that strain B-1 produced TTX and related substances; mouse bioassay revealed that 23.9 mouse units (MU) of toxins were present in 200ml of broth medium. On the basis of the physiological and biochemical characteristics of this strain and the results of 16S rRNA analysis, strain B-1 was identified as Lysinibacillus fusiformis.

摘要

从暗纹东方鲀肝脏中分离到 6 株优势菌，筛选其产 TTX 能力。电喷雾电离-质谱（ESI-MS）分析表明，菌株 B-1 产生 TTX 及相关物质；小鼠生物测定表明，在 200ml 发酵液中存在 23.9 鼠单位（MU）的毒素。根据该菌株的生理生化特性和 16S rRNA 分析结果，将菌株 B-1 鉴定为梭菌。