Mills Paul J, Hong Suzi, Redwine Laura, Carter Steven M, Chiu Albert, Ziegler Michael G, Dimsdale Joel E, Maisel Alan S
Department of Psychiatry, University of California, San Diego, La Jolla, CA, USA.
J Appl Physiol (1985). 2006 Sep;101(3):785-8. doi: 10.1152/japplphysiol.00135.2006. Epub 2006 May 25.
Studies suggest that physical fitness promotes cardiovascular health, including improved endothelial function and possibly reduced inflammatory responses to stressors. This study examined the effects of fitness on leukocyte-endothelial adhesion in response to an acute exercise challenge. Peripheral blood mononuclear cell (PBMC) adhesion to human umbilical venous endothelial cells (HUVEC) was examined in 18 more-fit and 19 less-fit individuals [mean age 39 yr (SD 11)] before and after a 20-min treadmill exercise at 65-70% peak oxygen consumption. PBMC were isolated from whole blood (Ficoll-Paque) at rest and immediately after exercise. HUVEC were incubated for 4 h in the presence of cytokines IL-1 and IL-8 to activate endothelial adhesion molecule expression. Fit subjects showed a significant reduction in PBMC-HUVEC adhesion after exercise (P < 0.01) compared with less-fit subjects, who showed no significant change. Regardless of fitness levels, both at rest and in response to exercise, soluble ICAM-1 in the incubation media attenuated PBMC-HUVEC adhesion by approximately 81% (P < 0.001). The findings indicate that immune cells that demarginate in response to exercise have reduced ability to adhere in individuals who are physically fit, an effect apparently independent of ICAM-1 binding. The findings provide evidence of how physical fitness might protect individuals from inflammatory responses to exercise.
研究表明，身体健康有助于促进心血管健康，包括改善内皮功能，并可能减少对应激源的炎症反应。本研究调查了健康状况对急性运动挑战后白细胞与内皮细胞黏附的影响。在18名身体状况较好和19名身体状况较差的个体[平均年龄39岁（标准差11）]中，于65 - 70%最大耗氧量下进行20分钟跑步机运动前后，检测外周血单核细胞（PBMC）与人脐静脉内皮细胞（HUVEC）的黏附情况。PBMC在静息时和运动后立即从全血（Ficoll-Paque）中分离出来。HUVEC在细胞因子IL-1和IL-8存在的情况下孵育4小时，以激活内皮黏附分子的表达。与身体状况较差的受试者相比，身体状况较好的受试者运动后PBMC-HUVEC黏附显著降低（P < 0.01），而身体状况较差的受试者无显著变化。无论健康水平如何，在静息时以及运动反应中，孵育培养基中的可溶性ICAM-1均可使PBMC-HUVEC黏附降低约81%（P < 0.001）。研究结果表明，在运动后从边缘移出的免疫细胞在身体健康的个体中黏附能力降低，这一效应显然与ICAM-1结合无关。这些发现为身体健康如何保护个体免受运动引发的炎症反应提供了证据。
