Barry A L, Reller L B, Miller G H, Washington J A, Schoenknect F D, Peterson L R, Hare R S, Knapp C
Clinical Microbiology Institute, Tualatin, Oregon 97062.
J Clin Microbiol. 1992 Mar;30(3):585-9. doi: 10.1128/jcm.30.3.585-589.1992.
A multilaboratory study was undertaken to reassess the amount of calcium and magnesium that should be added to Mueller-Hinton broth when testing Pseudomonas aeruginosa against amikacin, gentamicin, isepamicin, netilmicin, and tobramycin. To achieve parity with agar dilution tests, cation-adjusted broth should contain 20 to 25 mg of calcium and 10 to 12.5 mg of magnesium per liter rather than the 50- and 25-mg/liter supplements recommended previously. For quality control of tests with contemporary media, MIC control limits should be adjusted by lowering the current MIC limits by at least 1 doubling-dilution interval.
开展了一项多实验室研究，以重新评估在对铜绿假单胞菌进行阿米卡星、庆大霉素、异帕米星、奈替米星和妥布霉素药敏试验时，应添加到 Mueller-Hinton 肉汤中的钙和镁的量。为了与琼脂稀释试验取得一致，阳离子调整肉汤每升应含有 20 至 25 毫克钙和 10 至 12.5 毫克镁，而不是先前推荐的 50 毫克/升和 25 毫克/升的添加量。对于使用当代培养基进行试验的质量控制，应通过将当前的 MIC 限值至少降低 1 个双倍稀释间隔来调整 MIC 控制限。
