Germanà A, Montalbano G, Laurà R, Ciriaco E, del Valle M E, Vega José A
Dipartimento di Morfologia, Biochimica, Fisiologia e Produzioni Animali, Sezione di Morfologia, Università di Messina, Messina, Italy.
Neurosci Lett. 2004 Nov 23;371(2-3):196-8. doi: 10.1016/j.neulet.2004.08.077.
The olfactory epithelium of some teleosts, including zebrafish, contains three types of olfactory sensory neurons. Because zebrafish has become an ideal model for the study of neurogenesis in the olfactory system, it is of capital importance the identification of specific markers for different neuronal populations. In this study we used immunohistochemistry to analyze the distribution of S100 protein-like in the adult zebrafish olfactory epithelium. Surprisingly, specific S100 protein-like immunostaining was detected exclusively in crypt neurons, whereas ciliated and microvillous neurons were not reactive, and the supporting glial cells as well. The pattern of immunostaining was exclusively cytoplasmic without apparent polarity within the soma, and the intensity of immunostaining was not related with the maturative stage of the neurons. The role of S100 protein in crypt olfactory neurons is unknown, although it is probably associated with the capacity of these cells to respond to chemical stimuli. In any case, it represents an excellent marker to identify crypt olfactory neurons in zebrafish.
包括斑马鱼在内的一些硬骨鱼的嗅觉上皮包含三种类型的嗅觉感觉神经元。由于斑马鱼已成为研究嗅觉系统神经发生的理想模型，因此识别不同神经元群体的特异性标记至关重要。在本研究中，我们使用免疫组织化学分析了成年斑马鱼嗅觉上皮中S100蛋白样物质的分布。令人惊讶的是，仅在隐窝神经元中检测到特异性S100蛋白样免疫染色，而纤毛神经元和微绒毛神经元以及支持性神经胶质细胞均无反应。免疫染色模式仅为细胞质，在胞体内无明显极性，且免疫染色强度与神经元的成熟阶段无关。S100蛋白在隐窝嗅觉神经元中的作用尚不清楚，尽管它可能与这些细胞对化学刺激的反应能力有关。无论如何，它是识别斑马鱼隐窝嗅觉神经元的优秀标记。
