White E J, Kounelis S K, Byrd-Jacobs C A
Department of Biological Sciences, Western Michigan University, 1903 West Michigan Avenue, Kalamazoo, MI 49008-5410, USA.
Neuroscience. 2015 Jan 22;284:622-631. doi: 10.1016/j.neuroscience.2014.10.036. Epub 2014 Oct 30.
The zebrafish olfactory system is a valuable model for examining neural regeneration after damage due to the remarkable plasticity of this sensory system and of fish species. We applied detergent to the olfactory organ and examined the effects on both morphology and function of the olfactory system in adult zebrafish. Olfactory organs were treated once with Triton X-100 unilaterally to study glomerular innervation patterns or bilaterally to study odor detection. Fish were allowed to recover for 4-10 days and were compared to untreated control fish. Axonal projections were analyzed using whole mount immunocytochemistry with anti-keyhole limpet hemocyanin, a marker of olfactory axons in teleosts. Chemical lesioning of the olfactory organ with a single dose of Triton X-100 had profound effects on glomerular distribution in the olfactory bulb at 4 days after treatment, with the most significant effects in the medial region of the bulb. Glomeruli had returned by 7 days post-treatment. Analysis of the ability of the fish to detect cocktails of amino acids or bile salts consisted of counting the number of turns the fish made before and after odorant delivery. Control fish turned more after exposure to both odorants. Fish tested 4 and 7 days after chemical lesioning made more turns in response to amino acids but did not respond to bile salts. At 10 days post-lesion, these fish had regained the ability to detect bile salts. Thus, the changes seen in bulbar innervation patterns correlated to odorant-mediated behavior. We show that the adult zebrafish brain has the capacity to recover rapidly from detergent damage of the olfactory epithelium, with both glomerular distribution and odorant-mediated behavior returning in 10 days.
斑马鱼嗅觉系统是研究损伤后神经再生的一个有价值的模型，因为这种感官系统和鱼类物种具有显著的可塑性。我们将去污剂应用于嗅觉器官，并研究其对成年斑马鱼嗅觉系统形态和功能的影响。为了研究肾小球神经支配模式，单侧用 Triton X - 100 处理嗅觉器官一次；为了研究气味检测，则双侧处理。让鱼恢复 4 - 10 天，并与未处理的对照鱼进行比较。使用抗钥孔血蓝蛋白的全组织免疫细胞化学分析轴突投射，抗钥孔血蓝蛋白是硬骨鱼嗅觉轴突的一种标记物。用单剂量 Triton X - 100 对嗅觉器官进行化学损伤，在处理后 4 天对嗅球中的肾小球分布有深远影响，并在嗅球内侧区域影响最为显著。处理后 7 天肾小球已恢复。分析鱼检测氨基酸或胆汁盐混合物的能力包括计算在气味剂递送前后鱼转动的次数。对照鱼在接触两种气味剂后转动更多。化学损伤后 4 天和 7 天测试的鱼对氨基酸反应时转动更多，但对胆汁盐无反应。在损伤后 10 天，这些鱼恢复了检测胆汁盐的能力。因此，在嗅球神经支配模式中看到的变化与气味剂介导的行为相关。我们表明，成年斑马鱼大脑有能力从嗅觉上皮的去污剂损伤中迅速恢复，肾小球分布和气味剂介导的行为在 10 天内均可恢复。
