On S L, Holmes B
Identification Services Laboratory, National Collection of Type Cultures, London, United Kingdom.
J Clin Microbiol. 1992 Mar;30(3):746-9. doi: 10.1128/jcm.30.3.746-749.1992.
Twenty-one type or other reference strains, each representing a different Campylobacter, Helicobacter, or Arcobacter taxon, and a reference strain of Staphylococcus aureus were used to assess the reproducibility of nine enzyme detection tests used in the identification of campylobacters. For five of the tests (alkaline phosphatase, DNase, and H2S production, indoxyl acetate hydrolysis, and nitrate reduction), more than one procedure was employed to determine the most suitable method. Alkaline phosphatase test results were better defined and more reproducible if read after 1 h of incubation. Detection of DNase was fully reproducible with each method (except with Helicobacter pylori), but reactions were generally weaker than those of other DNase-producing organisms. Both procedures for determining H2S production were irreproducible for the same strains. The reproducibility of indoxyl acetate hydrolysis was improved by using disks impregnated with 25 microliters of substrate. Reduction of nitrate was best determined by Cook's plate method. Results for the other tests examined (catalase, oxidase, and urease production and hippurate hydrolysis) were both pertinent and fully reproducible for all strains.
使用21种类型或其他参考菌株，每种代表不同的弯曲杆菌属、幽门螺杆菌属或弓形杆菌属分类单元，以及一株金黄色葡萄球菌参考菌株，来评估用于弯曲杆菌鉴定的9种酶检测试验的可重复性。对于其中5种试验（碱性磷酸酶、DNA酶、H2S产生、吲哚乙酸水解和硝酸盐还原），采用了不止一种方法来确定最合适的方法。如果在培养1小时后读取碱性磷酸酶试验结果，则结果更明确且更具可重复性。每种方法检测DNA酶都具有完全可重复性（除幽门螺杆菌外），但反应通常比其他产生DNA酶的生物体弱。两种检测H2S产生的方法对相同菌株均不可重复。使用浸渍有25微升底物的滤纸片可提高吲哚乙酸水解的可重复性。硝酸盐还原最好用库克平板法测定。所检测的其他试验（过氧化氢酶、氧化酶、脲酶产生和马尿酸盐水解）的结果对所有菌株均相关且完全可重复。
