针对弯曲杆菌属及相关微生物的三项补充诊断测试。
Three supplementary diagnostic tests for Campylobacter species and related organisms.
作者信息
Burnens A P, Nicolet J
机构信息
Institute for Veterinary Bacteriology, University of Berne, Switzerland.
出版信息
J Clin Microbiol. 1993 Mar;31(3):708-10. doi: 10.1128/jcm.31.3.708-710.1993.
Abstract
We analyzed 307 recent clinical isolates and reference strains of Campylobacter spp., Helicobacter spp., and Arcobacter spp. for arylsulfatase and pyrazinamidase activities and susceptibility to polymyxin B. All isolates studied were identified by DNA dot hybridization prior to testing. Campylobacter concisus, C. sputorum, and Helicobacter fennelliae were positive for arylsulfatase. C. jejuni, C. coli, C. lari, C. upsaliensis, and C. concisus were positive for pyrazinamidase. Only Helicobacter spp. were resistant to polymyxin B. C. fetus subsp. fetus, C. hyointestinalis, C. mucosalis, and Arcobacter spp. were negative in all three tests. The tests were simple to perform and read, and with the exception of C. sputorum biovars, all isolates within a species gave consistently positive or negative results in all the assays. These three phenotypic tests may help refine current methods for phenotypic identification of Campylobacter spp. and related organisms in a reference laboratory setting.
摘要
我们分析了307株弯曲杆菌属、幽门螺杆菌属和弓形杆菌属的近期临床分离株及参考菌株，检测其芳基硫酸酯酶和吡嗪酰胺酶活性以及对多粘菌素B的敏感性。所有研究的分离株在检测前均通过DNA斑点杂交进行鉴定。简明弯曲杆菌、唾液弯曲杆菌和芬内尔幽门螺杆菌的芳基硫酸酯酶呈阳性。空肠弯曲杆菌、结肠弯曲杆菌、拉氏弯曲杆菌、乌普萨拉弯曲杆菌和简明弯曲杆菌的吡嗪酰胺酶呈阳性。仅幽门螺杆菌属对多粘菌素B耐药。胎儿弯曲杆菌胎儿亚种、猪肠弯曲杆菌、黏膜弯曲杆菌和弓形杆菌属在所有三项检测中均为阴性。这些检测操作和判读都很简单，除唾液弯曲杆菌生物变种外，同一物种内的所有分离株在所有检测中均给出一致的阳性或阴性结果。这三项表型检测可能有助于完善参考实验室环境中弯曲杆菌属及相关生物体表型鉴定的现有方法。
相似文献
1
Three supplementary diagnostic tests for Campylobacter species and related organisms.针对弯曲杆菌属及相关微生物的三项补充诊断测试。
J Clin Microbiol. 1993 Mar;31(3):708-10. doi: 10.1128/jcm.31.3.708-710.1993.
2
[Detection of pyrazinamidase activity for differentiation of Campylobacter, Arcobacter, and Helicobacter spp. by using a high-performance liquid chromatography method].[利用高效液相色谱法检测吡嗪酰胺酶活性以鉴别弯曲杆菌属、弓形杆菌属和幽门螺杆菌属]
Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi. 1999 Dec;10(2):111-6.
3
Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov.弯曲杆菌属、螺杆菌属和沃氏菌属分类的修订：属描述的修正及新属——弓形杆菌属的提议
Int J Syst Bacteriol. 1991 Jan;41(1):88-103. doi: 10.1099/00207713-41-1-88.
4
Differentiation of Campylobacter species by AFLP fingerprinting.通过扩增片段长度多态性指纹图谱对弯曲杆菌属菌种进行鉴别
Microbiology (Reading). 2001 Oct;147(Pt 10):2729-2737. doi: 10.1099/00221287-147-10-2729.
5
The genus Campylobacter: a decade of progress.弯曲杆菌属：十年进展
Clin Microbiol Rev. 1988 Apr;1(2):157-72. doi: 10.1128/CMR.1.2.157.
6
Multilocus sequence typing methods for the emerging Campylobacter Species C. hyointestinalis, C. lanienae, C. sputorum, C. concisus, and C. curvus.用于新兴弯曲杆菌属物种 C. hyointestinalis、C. lanienae、C. sputorum、C. concisus 和 C. curvus 的多位点序列分型方法。
Front Cell Infect Microbiol. 2012 Apr 2;2:45. doi: 10.3389/fcimb.2012.00045. eCollection 2012.
7
Identification of thermotolerant campylobacter species by fluorescence in situ hybridization.通过荧光原位杂交鉴定耐热弯曲杆菌属菌种
J Clin Microbiol. 2008 Jun;46(6):2133-6. doi: 10.1128/JCM.01512-07. Epub 2008 Apr 2.
8
Fumarate hydration test for differentiation of Campylobacter and Helicobacter species.用于弯曲杆菌属和螺杆菌属菌种鉴别的延胡索酸水合试验
Kansenshogaku Zasshi. 1995 Jun;69(6):666-72. doi: 10.11150/kansenshogakuzasshi1970.69.666.
9
30 years of campylobacters: biochemical characteristics and a biotyping proposal for Campylobacter jejuni.弯曲杆菌研究30年：空肠弯曲杆菌的生化特性及生物分型建议
J Clin Microbiol. 1982 Jun;15(6):1065-73. doi: 10.1128/jcm.15.6.1065-1073.1982.
10
The possible relationship between Campylobacter spp./Arcobacter spp. and patients with ulcerative colitis.弯曲杆菌属/弓形杆菌属与溃疡性结肠炎患者之间的可能关系。
Eur J Gastroenterol Hepatol. 2018 May;30(5):531-538. doi: 10.1097/MEG.0000000000001095.
引用本文的文献
1
Temporal prevalence of antimicrobial resistance in Campylobacter spp. from beef cattle in Alberta feedlots.艾伯塔省饲养场肉牛弯曲杆菌属中抗菌药物耐药性的时间流行情况。
Appl Environ Microbiol. 2006 Jun;72(6):4088-95. doi: 10.1128/AEM.02830-05.
2
Isolation and identification of Helicobacter spp. from canine and feline gastric mucosa.从犬猫胃黏膜中分离和鉴定幽门螺旋杆菌属细菌。
Appl Environ Microbiol. 1998 Oct;64(10):3998-4006. doi: 10.1128/AEM.64.10.3998-4006.1998.
3
Polyphasic taxonomy, a consensus approach to bacterial systematics.多相分类学，一种细菌系统分类学的共识方法。
Microbiol Rev. 1996 Jun;60(2):407-38. doi: 10.1128/mr.60.2.407-438.1996.
4
Rapid identification of Campylobacter species by restriction fragment length polymorphism analysis of a PCR-amplified fragment of the gene coding for 16S rRNA.通过对编码16S rRNA的基因的PCR扩增片段进行限制性片段长度多态性分析快速鉴定弯曲杆菌属菌种。
J Clin Microbiol. 1996 Jan;34(1):62-7. doi: 10.1128/jcm.34.1.62-67.1996.
5
Molecular cloning and site-specific mutagenesis of a gene involved in arylsulfatase production in Campylobacter jejuni.空肠弯曲菌中参与芳基硫酸酯酶产生的基因的分子克隆和位点特异性诱变。
J Bacteriol. 1996 Jun;178(11):3335-8. doi: 10.1128/jb.178.11.3335-3338.1996.
6
Genotypic and phenotypic characterization of Helicobacter cinaedi and Helicobacter fennelliae strains isolated from humans and animals.从人和动物中分离出的辛内氏螺杆菌和芬内利螺杆菌菌株的基因型和表型特征
J Clin Microbiol. 1995 Nov;33(11):2940-7. doi: 10.1128/jcm.33.11.2940-2947.1995.
7
Novel Campylobacter-like organism resembling Helicobacter fennelliae isolated from a boy with gastroenteritis and from dogs.从一名患肠胃炎的男孩和狗身上分离出的类似芬内尔螺杆菌的新型弯曲菌样生物体。
J Clin Microbiol. 1993 Jul;31(7):1916-7. doi: 10.1128/jcm.31.7.1916-1917.1993.
8
Additional data on clinical isolates of Campylobacter mucosalis.关于黏液弯曲杆菌临床分离株的更多数据。
J Clin Microbiol. 1994 Sep;32(9):2338-9. doi: 10.1128/jcm.32.9.2338-2339.1994.
9
Bias of phenotypic techniques for identification of Campylobacter isolates.
Eur J Clin Microbiol Infect Dis. 1995 Feb;14(2):153-5. doi: 10.1007/BF02111881.
本文引用的文献
1
30 years of campylobacters: biochemical characteristics and a biotyping proposal for Campylobacter jejuni.弯曲杆菌研究30年：空肠弯曲杆菌的生化特性及生物分型建议
J Clin Microbiol. 1982 Jun;15(6):1065-73. doi: 10.1128/jcm.15.6.1065-1073.1982.
2
Epidemiology of Campylobacter jejuni infections.空肠弯曲菌感染的流行病学
Epidemiol Rev. 1983;5:157-76. doi: 10.1093/oxfordjournals.epirev.a036256.
3
Pyrazinamide susceptibility and amidase activity of tubercle bacilli.结核杆菌的吡嗪酰胺敏感性及酰胺酶活性
Am Rev Respir Dis. 1967 Mar;95(3):461-9. doi: 10.1164/arrd.1967.95.3.461.
4
Pyrazinamidase activity in Yersinia enterocolitica and related organisms.小肠结肠炎耶尔森菌及相关菌中的吡嗪酰胺酶活性
J Clin Microbiol. 1985 Jun;21(6):980-2. doi: 10.1128/jcm.21.6.980-982.1985.
5
The genus Campylobacter: a decade of progress.弯曲杆菌属：十年进展
Clin Microbiol Rev. 1988 Apr;1(2):157-72. doi: 10.1128/CMR.1.2.157.
6
Identification of EF group 22 campylobacters from gastroenteritis cases as Campylobacter concisus.从肠胃炎病例中鉴定出22组弯曲杆菌属为空肠弯曲菌。
J Clin Microbiol. 1989 Aug;27(8):1775-81. doi: 10.1128/jcm.27.8.1775-1781.1989.
7
"Campylobacter upsaliensis" isolated from blood cultures of pediatric patients.从儿科患者血培养物中分离出的“乌普萨拉弯曲菌”
J Clin Microbiol. 1989 Apr;27(4):657-9. doi: 10.1128/jcm.27.4.657-659.1989.
8
Campylobacter-Wolinella group organisms are the only oral bacteria that form arylsulfatase-active colonies on a synthetic indicator medium.弯曲杆菌-沃氏菌属菌群是唯一能在合成指示培养基上形成具有芳基硫酸酯酶活性菌落的口腔细菌。
Infect Immun. 1989 May;57(5):1380-3. doi: 10.1128/iai.57.5.1380-1383.1989.
9
Pyrazinamidase activity as a phenotypic marker for several Aeromonas spp. isolated from clinical specimens.吡嗪酰胺酶活性作为从临床标本中分离出的几种气单胞菌属细菌的表型标志物。
J Clin Microbiol. 1990 Feb;28(2):391-2. doi: 10.1128/jcm.28.2.391-392.1990.
10
Evaluation of the indoxyl acetate hydrolysis test for rapid differentiation of Campylobacter, Helicobacter, and Wolinella species.用于弯曲杆菌、幽门螺杆菌和沃氏嗜胆菌属菌种快速鉴别的吲哚乙酸水解试验评估
J Clin Microbiol. 1990 Oct;28(10):2335-9. doi: 10.1128/jcm.28.10.2335-2339.1990.
Zotero 插件