Burnens A P, Nicolet J
Institute for Veterinary Bacteriology, University of Berne, Switzerland.
J Clin Microbiol. 1993 Mar;31(3):708-10. doi: 10.1128/jcm.31.3.708-710.1993.
We analyzed 307 recent clinical isolates and reference strains of Campylobacter spp., Helicobacter spp., and Arcobacter spp. for arylsulfatase and pyrazinamidase activities and susceptibility to polymyxin B. All isolates studied were identified by DNA dot hybridization prior to testing. Campylobacter concisus, C. sputorum, and Helicobacter fennelliae were positive for arylsulfatase. C. jejuni, C. coli, C. lari, C. upsaliensis, and C. concisus were positive for pyrazinamidase. Only Helicobacter spp. were resistant to polymyxin B. C. fetus subsp. fetus, C. hyointestinalis, C. mucosalis, and Arcobacter spp. were negative in all three tests. The tests were simple to perform and read, and with the exception of C. sputorum biovars, all isolates within a species gave consistently positive or negative results in all the assays. These three phenotypic tests may help refine current methods for phenotypic identification of Campylobacter spp. and related organisms in a reference laboratory setting.
我们分析了307株弯曲杆菌属、幽门螺杆菌属和弓形杆菌属的近期临床分离株及参考菌株，检测其芳基硫酸酯酶和吡嗪酰胺酶活性以及对多粘菌素B的敏感性。所有研究的分离株在检测前均通过DNA斑点杂交进行鉴定。简明弯曲杆菌、唾液弯曲杆菌和芬内尔幽门螺杆菌的芳基硫酸酯酶呈阳性。空肠弯曲杆菌、结肠弯曲杆菌、拉氏弯曲杆菌、乌普萨拉弯曲杆菌和简明弯曲杆菌的吡嗪酰胺酶呈阳性。仅幽门螺杆菌属对多粘菌素B耐药。胎儿弯曲杆菌胎儿亚种、猪肠弯曲杆菌、黏膜弯曲杆菌和弓形杆菌属在所有三项检测中均为阴性。这些检测操作和判读都很简单，除唾液弯曲杆菌生物变种外，同一物种内的所有分离株在所有检测中均给出一致的阳性或阴性结果。这三项表型检测可能有助于完善参考实验室环境中弯曲杆菌属及相关生物体表型鉴定的现有方法。
