Jammes Yves, Steinberg Jean Guillaume, Brégeon Fabienne, Delliaux Stéphane
Service des Explorations Fonctionnelles Respiratoires, Hôpital Nord, Assistance Publique-Hôpitaux de Marseille, Chemin des Bourrely, 13015 Marseille, France.
Respir Physiol Neurobiol. 2004 Nov 30;144(1):81-90. doi: 10.1016/j.resp.2004.07.015.
The kinetics of blood markers of the oxidative stress during and after an incremental exercise until the maximal performances is not documented in healthy sedentary subjects. We studied subjects of both sexes cycling on an ergometer until or near the V(O)(2)(max) measurement, and we measured during exercise and a 30-min recovery period the plasma concentration of thiobarbituric acid reactive substances (TBARS) which explored the production of reactive oxygen species (ROS) and two antioxidants (plasma reduced ascorbic acid (RAA) and erythrocyte reduced glutathione (GSH)). Despite we noted inter-individual differences in the instants of maximal variations of TBARS, GSH, and RAA, they were all measured within the first 20 min of the post-exercise recovery period, and at the 30th min of recovery, the three ROS blood markers tended to recover their pre-exercise levels. The maximal TBARS increase was positively correlated with V(O)(2)(max) and negatively correlated with the magnitude of RAA consumption. Our results indicate the existence of an early post-exercise oxidative stress in healthy sedentary volunteers. They also show that the ROS production is proportional to the maximal aerobic power and inversely related to the consumption of plasma antioxidants.
在健康久坐不动的受试者中，关于递增运动直至达到最大运动能力期间及之后氧化应激的血液标志物动力学情况尚无文献记载。我们研究了不同性别的受试者在测力计上进行骑行运动直至或接近测量最大摄氧量（V(O)(2)(max)），并在运动期间以及30分钟的恢复期内测量了硫代巴比妥酸反应性物质（TBARS）的血浆浓度，该物质可反映活性氧（ROS）的产生情况，同时还测量了两种抗氧化剂（血浆还原型抗坏血酸（RAA）和红细胞还原型谷胱甘肽（GSH））。尽管我们注意到TBARS、GSH和RAA最大变化时刻存在个体差异，但它们均在运动后恢复期的前20分钟内测得，且在恢复的第30分钟时，这三种ROS血液标志物趋于恢复到运动前水平。TBARS的最大增幅与V(O)(2)(max)呈正相关，与RAA消耗幅度呈负相关。我们的结果表明，健康久坐志愿者在运动后早期存在氧化应激。这些结果还表明，ROS的产生与最大有氧能力成正比，与血浆抗氧化剂的消耗呈反比。
