Yu Li, Li Qing-wei, Ryder O A, Zhang Ya-ping
Laboratory of Molecular Biology of Domestic Animals, and Cellular and Molecular Evolution, Kunming Institute of Zoology, Kunming 650223, China.
Mol Phylogenet Evol. 2004 Dec;33(3):694-705. doi: 10.1016/j.ympev.2004.08.001.
Phylogenetic relationships among 37 living species of order Carnivora spanning a relatively broad range of divergence times and taxonomic levels were examined using nuclear sequence data from exon 1 of the IRBP gene (approximately 1.3 kb) and first intron of the TTR gene (approximately 1 kb). These data were used to analyze carnivoran phylogeny at the family and generic level as well as the interspecific relationships within recently derived Felidae. Phylogenetic results using a combined IRBP+TTR dataset strongly supported within the superfamily Califormia, the red panda as the closest lineage to procyonid-mustelid (i.e., Musteloidea) clade followed by pinnipeds (Otariidae and Phocidae), Ursidae (including the giant panda), and Canidae. Four feliform families, namely the monophyletic Herpestidae, Hyaenidae, and Felidae, as well as the paraphyletic Viverridae were consistently recovered convincingly. The utilities of these two gene segments for the phylogenetic analyses were extensively explored and both were found to be fairly informative for higher-group associations within the order Carnivora, but not for those of low level divergence at the species level. Therefore, there is a need to find additional genetic markers with more rapid mutation rates that would be diagnostic at deciphering relatively recent relationships within the Carnivora.
利用来自IRBP基因第1外显子（约1.3 kb）和TTR基因第1内含子（约1 kb）的核序列数据，研究了37种食肉目现存物种之间的系统发育关系，这些物种跨越了相对广泛的分歧时间和分类水平。这些数据被用于分析食肉目在科和属水平上的系统发育，以及最近分化出的猫科动物种间关系。使用IRBP + TTR组合数据集的系统发育结果有力地支持了在犬型亚目内，小熊猫是最接近浣熊 - 鼬科（即鼬超科）进化枝的谱系，其次是鳍足类（海狮科和海豹科）、熊科（包括大熊猫）和犬科。四个猫型总科家族，即单系的獴科、鬣狗科和猫科，以及并系的灵猫科，都始终如一地令人信服地被恢复。广泛探索了这两个基因片段在系统发育分析中的效用，发现它们对于食肉目内较高分类群的关联相当有信息价值，但对于物种水平的低水平分化则不然。因此，有必要找到额外的具有更快突变率的遗传标记，以便在解读食肉目内相对较近的关系时具有诊断性。
