从墨西哥湾海水和鱼产品中分离出的副溶血性弧菌菌株的血清学和分子特征
Serologic and molecular characterization of Vibrio parahaemolyticus strains isolated from seawater and fish products of the Gulf of Mexico.
作者信息
Cabrera-García María Eugenia, Vázquez-Salinas Carlos, Quiñones-Ramírez Elsa Irma
机构信息
Department of Microbiology of the National School of Biological Sciences, Instituto Politécnico Nacional, Mexico City, Mexico.
出版信息
Appl Environ Microbiol. 2004 Nov;70(11):6401-6. doi: 10.1128/AEM.70.11.6401-6406.2004.
Abstract
The thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) are the main virulence factors of Vibrio parahaemolyticus. We isolated V. parahaemolyticus from seawater, fish, and oysters obtained from the Pueblo Viejo Lagoon in Veracruz, determined the serogroups, phenotypically and genotypically characterized TDH and TRH, and investigated the presence of the toxR gene. A total of 46 V. parahaemolyticus strains were isolated, and all of them amplified the 368-bp toxR gene fragment. The trh gene was not identified in any of the strains; 4 of the 46 strains were Kanagawa phenomenon (KP) positive and amplified the 251-bp tdh gene fragment. The most frequent serogroup was serogroup O3. This is the first report of the presence of KP-positive tdh-positive environmental V. parahaemolyticus strains in Mexico.
摘要
耐热直接溶血素（TDH）和TDH相关溶血素（TRH）是副溶血性弧菌的主要毒力因子。我们从韦拉克鲁斯州普韦布洛·维埃霍泻湖采集的海水、鱼类和牡蛎中分离出副溶血性弧菌，确定其血清群，从表型和基因型上对TDH和TRH进行特征分析，并研究toxR基因的存在情况。共分离出46株副溶血性弧菌菌株，所有菌株均扩增出368 bp的toxR基因片段。在任何菌株中均未鉴定出trh基因；46株菌株中有4株神奈川现象（KP）呈阳性，并扩增出251 bp的tdh基因片段。最常见的血清群是O3血清群。这是墨西哥首次报道存在KP阳性、tdh阳性的环境副溶血性弧菌菌株。
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