评估LIVE/DEAD BacLight试剂盒用于检测嗜极端古菌及可视化环境高盐样品中的微生物。
Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples.
作者信息
Leuko Stefan, Legat Andrea, Fendrihan Sergiu, Stan-Lotter Helga
机构信息
Cyanobacteria and Astrobiology Research Laboratory, University of New South Wales, Sydney, Australia.
出版信息
Appl Environ Microbiol. 2004 Nov;70(11):6884-6. doi: 10.1128/AEM.70.11.6884-6886.2004.
Abstract
Extremophilic archaea were stained with the LIVE/DEAD BacLight kit under conditions of high ionic strength and over a pH range of 2.0 to 9.3. The reliability of the kit was tested with haloarchaea following permeabilization of the cells. Microorganisms in hypersaline environmental samples were detectable with the kit, which suggests its potential application to future extraterrestrial halites.
摘要
嗜极端古菌在高离子强度条件下以及pH值范围为2.0至9.3的环境中，使用LIVE/DEAD BacLight试剂盒进行染色。在细胞透化处理后，使用该试剂盒对嗜盐古菌进行测试，以检验其可靠性。该试剂盒可检测高盐环境样品中的微生物，这表明其在未来外星卤化物研究中的潜在应用价值。
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