Gerlach Gabriele, Janzen Simone, Beier Dagmar, Gross Roy
Theodor-Boveri-Institut für Biowissenschaften, Lehrstuhl für Mikrobiologie, Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany.
Microbiology (Reading). 2004 Nov;150(Pt 11):3715-3729. doi: 10.1099/mic.0.27432-0.
The BvgAS two-component system is the master regulator of virulence gene expression in the mammalian pathogens Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. This paper reports the partial cloning and characterization of the bvgAS loci of the 'new' Bordetella species Bordetella holmesii, Bordetella trematum and Bordetella hinzii, which are increasingly recognized as opportunistic pathogens in humans. It is demonstrated that the cytoplasmic signalling domains of the BvgS histidine kinases of B. pertussis and B. holmesii are functionally interchangeable, while signal perception by the two sensor proteins seems to be different. Furthermore, it is shown that, despite the high similarity of the BvgA proteins of B. pertussis and B. holmesii, promoter recognition by the response regulator proteins differs substantially in these organisms.
BvgAS双组分系统是哺乳动物病原体百日咳博德特氏菌、副百日咳博德特氏菌和支气管败血博德特氏菌中毒力基因表达的主要调节因子。本文报道了“新”博德特氏菌属物种霍氏博德特氏菌、特氏博德特氏菌和欣氏博德特氏菌的bvgAS基因座的部分克隆及特性分析，这些菌越来越被认为是人类的机会致病菌。结果表明，百日咳博德特氏菌和霍氏博德特氏菌的BvgS组氨酸激酶的细胞质信号结构域在功能上是可互换的，而两种传感蛋白的信号感知似乎有所不同。此外，研究表明，尽管百日咳博德特氏菌和霍氏博德特氏菌的BvgA蛋白高度相似，但在这些生物体中，反应调节蛋白对启动子的识别却有很大差异。
