Tsai Hsiang-Jung, Tseng Chun-hsien, Chang Poa-chun, Mei Kai, Wang Shih-Chi
Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei 106, Taiwan.
Avian Dis. 2004 Sep;48(3):512-21. doi: 10.1637/7172.
To understand the genetic variations between the field strains of waterfowl parvoviruses and their attenuated derivatives, we analyzed the complete nucleotide sequences of the viral protein 1 (VP1) genes of nine field strains and two vaccine strains of waterfowl parvoviruses. Sequence comparison of the VP1 proteins showed that these viruses could be divided into goose parvovirus (GPV) related and Muscovy duck parvovirus (MDPV) related groups. The amino acid difference between GPV- and MDPV-related groups ranged from 13.1% to 15.8%, and the most variable region resided in the N terminus of VP2. The vaccine strains of GPV and MDPV exhibited only 1.2% and 0.3% difference in amino acid when compared with their parental field strains, and most of these differences resided in residues 497-575 of VP1, suggesting that these residues might be important for the attenuation of GPV and MDPV. When the GPV strains isolated in 1982 (the strain 82-0308) and in 2001 (the strain 01-1001) were compared, only 0.3% difference in amino acid was found, while MDPV strains isolated in 1990 (the strain 90-0219) and 1997 (the strain 97-0104) showed only 0.4% difference in amino acid. The result indicates that the genome of waterfowl parvovirus had remained highly stable in the field.
为了解水禽细小病毒野毒株及其减毒衍生物之间的遗传变异，我们分析了9株水禽细小病毒野毒株和2株疫苗株的病毒蛋白1（VP1）基因的完整核苷酸序列。VP1蛋白的序列比较表明，这些病毒可分为鹅细小病毒（GPV）相关组和番鸭细小病毒（MDPV）相关组。GPV相关组和MDPV相关组之间的氨基酸差异范围为13.1%至15.8%，最可变区位于VP2的N端。GPV和MDPV的疫苗株与其亲本野毒株相比，氨基酸差异仅为1.2%和0.3%，且这些差异大多位于VP1的497-575位残基，这表明这些残基可能对GPV和MDPV的减毒很重要。当比较1982年分离的GPV毒株（82-0308株）和2001年分离的毒株（01-1001株）时，发现氨基酸差异仅为0.3%，而1990年分离（90-0219株）和1997年分离（97-0104株）的MDPV毒株氨基酸差异仅为0.4%。结果表明，水禽细小病毒的基因组在野外保持高度稳定。
