Vega-Maray Ana M, Fernández-González Delia, Valencia-Barrera Rosa, Polo Florentino, Seoane-Camba Juan A, Sudrez-Cervera Maria
Department of Plant Biology, Faculty of Biology, University of León, León, Spain.
Eur J Cell Biol. 2004 Sep;83(9):493-7. doi: 10.1078/0171-9335-00406.
Parietariajudaica L. (Urticaceae) pollen is considered one of the most common causes of allergic respiratory symptoms in the Mediterranean area. The localization of lipid transfer proteins (LTPs) in P. judaica mature and hydrated-activated pollen grains was investigated applying a combination of transmission electron microscopy (TEM) with immunocytochemical methods. Our results show that the content of LTPs in P. judaica pollen grains changes during the process of hydration. The localization of judaica LTPs in the cytoplasm and in the lipid bodies associated with vacuoles demonstrated that LTPs represent primarily intracellular proteins. On the other hand, exposure of the pollen grains to germination medium induced the release of LTPs from the pollen grain. Thus, LTPs are cytoplasmic proteins that are secreted to become available for pollen-stigma interactions and probably induce the IgE antibody responses in allergic patients.
墙草（荨麻科）花粉被认为是地中海地区过敏性呼吸道症状最常见的病因之一。采用透射电子显微镜（TEM）与免疫细胞化学方法相结合的手段，研究了墙草成熟及水合激活花粉粒中脂质转移蛋白（LTPs）的定位。我们的结果表明，墙草花粉粒中LTPs的含量在水合过程中会发生变化。墙草LTPs在细胞质以及与液泡相关的脂质体中的定位表明，LTPs主要代表细胞内蛋白。另一方面，将花粉粒暴露于萌发培养基会诱导LTPs从花粉粒中释放。因此，LTPs是细胞质蛋白，它们被分泌出来以参与花粉-柱头相互作用，并且可能在过敏患者中诱导IgE抗体反应。
