Lim Jee-Min, Yoon Jung-Hoon, Lee Jae-Chan, Jeon Che Ok, Park Dong-Jin, Sung Changkeun, Kim Chang-Jin
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Department of Food Science and Technology, Chungnam University, Daejeon 305-764, Republic of Korea.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2037-2042. doi: 10.1099/ijs.0.63194-0.
A moderately halophilic bacterium, strain SS20(T), capable of growing at salinities of 1-20 % (w/v) NaCl was isolated from a solar saltern of the Dangjin area in Korea and was characterized taxonomically. Strain SS20(T) was a Gram-negative bacterium comprising motile, short rods. Its major cellular fatty acids were C(18 : 1)omega7c, C(19 : 0)omega8c cyclo and C(16 : 0). The DNA G+C content was 70 mol% and the predominant ubiquinone was Q-9. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SS20(T) belonged to the genus Halomonas. The levels of 16S rRNA gene sequence similarity to the type strains of Halomonas species were in the range 93.0-97.5 %. The levels of DNA-DNA relatedness between strain SS20(T) and the type strains of phylogenetically closely related Halomonas species were in the range 5.3-12.3 %. On the basis of physiological and molecular properties, strain SS20(T) represents a novel species of the genus Halomonas, for which the name Halomonas koreensis sp. nov. is proposed. The type strain is SS20(T) (=KCTC 12127(T)=JCM 12237(T)).
从韩国唐津地区的一个盐场分离出了一株中度嗜盐细菌，菌株SS20(T)，它能够在1-20%（w/v）NaCl的盐度下生长，并进行了分类学特征分析。菌株SS20(T)是革兰氏阴性菌，由运动性短杆菌组成。其主要细胞脂肪酸为C(18 : 1)ω7c、C(19 : 0)ω8c环丙烷和C(16 : 0)。DNA G+C含量为70 mol%，主要泛醌为Q-9。基于16S rRNA基因序列的系统发育分析表明，菌株SS20(T)属于嗜盐单胞菌属。与嗜盐单胞菌属模式菌株的16S rRNA基因序列相似性水平在93.0-97.5%范围内。菌株SS20(T)与系统发育密切相关的嗜盐单胞菌属模式菌株之间的DNA-DNA相关性水平在5.3-12.3%范围内。基于生理和分子特性，菌株SS20(T)代表嗜盐单胞菌属的一个新物种，为此提出了嗜盐韩国单胞菌（Halomonas koreensis sp. nov.）的名称。模式菌株为SS20(T)（=KCTC 12127(T)=JCM 12237(T)）。
