Lee Jae-Chan, Jeon Che Ok, Lim Jee-Min, Lee Sang-Mi, Lee Jung-Min, Song Sung-Min, Park Dong-Jin, Li Wen-Jun, Kim Chang-Jin
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Environmental Biotechnology National Core Research Center, Division of Environmental Biotechnology, Gyeongsang National University, 660-701, Republic of Korea.
Int J Syst Evol Microbiol. 2005 Sep;55(Pt 5):2027-2032. doi: 10.1099/ijs.0.63616-0.
A moderately halophilic, Gram-negative bacterium, strain BH539(T), which was isolated from a solar saltern at Taean in Korea, was considered to be a member of the genus Halomonas. Strain BH539(T) grew at salinities of 1-25% (w/v) and at temperatures of 10-45 degrees C. Cells were short rods that were motile by means of several flagella. Their major fatty acids were C(18:1)omega7c, C(16:0) and C(19:0) cyclo omega8c. The genomic DNA G+C content was about 65 mol% and the predominant ubiquinone was Q-9. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate formed a branch of the species Cobetia marina. However, 23S and 16S rRNA gene sequence similarities revealed that strain BH539(T) was related more closely to the type strains of the genus Halomonas. Phylogenetic analyses based on 23S rRNA gene sequences also indicated that the strain formed a phyletic line within the genus Halomonas. Therefore, it was concluded that strain BH539(T) should be classified within the genus Halomonas, rather than Cobetia. On the basis of physiological and molecular properties, strain BH539(T) represents a novel species of the genus Halomonas, for which the name Halomonas taeanensis sp. nov. is proposed. The type strain is BH539(T) (=KCTC 12284(T)=DSM 16463(T)).
从韩国泰安的一个太阳能盐场分离出的一株中度嗜盐革兰氏阴性细菌BH539(T)，被认为是嗜盐单胞菌属的成员。BH539(T)菌株在1-25%（w/v）的盐度和10-45℃的温度下生长。细胞为短杆状，借助几根鞭毛运动。其主要脂肪酸为C(18:1)ω7c、C(16:0)和C(19:0)环ω8c。基因组DNA的G+C含量约为65 mol%，主要泛醌为Q-9。基于16S rRNA基因序列的系统发育分析表明，该分离株形成了海洋科贝氏菌物种的一个分支。然而，23S和16S rRNA基因序列相似性表明，BH539(T)菌株与嗜盐单胞菌属的模式菌株关系更密切。基于23S rRNA基因序列的系统发育分析也表明，该菌株在嗜盐单胞菌属内形成了一个进化谱系。因此，得出结论，BH539(T)菌株应归类于嗜盐单胞菌属，而非科贝氏菌属。基于生理和分子特性，BH539(T)菌株代表嗜盐单胞菌属的一个新物种，为此提出名为泰安嗜盐单胞菌（Halomonas taeanensis sp. nov.）。模式菌株为BH539(T)（=KCTC 12284(T)=DSM 16463(T)）。
