Wang Ya-Nan, Cai Hua, Yu Su-Lin, Wang Zhi-Yao, Liu Jie, Wu Xiao-Lei
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Daqing Oilfield Company Ltd, Daqing 163712, China.
Int J Syst Evol Microbiol. 2007 May;57(Pt 5):911-915. doi: 10.1099/ijs.0.64826-0.
Two moderately halophilic strains, SL014B-69(T) and SL014B-62A2, were isolated from a saline soil contaminated with crude oil in Gudao in the coastal Shengli oilfield in China; the isolates were Gram-negative, rod-shaped and carried lateral flagella. Growth occurred at NaCl concentrations of 1-20 % (w/v), at temperatures of 10-42 degrees C and at pH 8.0-9.0. Strain SL014B-69(T) had C(18 : 1)omega7c (28.61 %), C(19 : 1) cyclo omega7c (27.97 %), C(16 : 0) (19.66 %) and C(12 : 0) 3-OH (8.87 %) as the predominant fatty acids and Q9 as the major ubiquinone, with the G+C content of genomic DNA being 64.0 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains belonged to genus of Halomonas in the Gammaproteobacteria, with the highest 16S rRNA gene sequence similarities of 96.4 % with Halomonas campisalis ATCC 700597(T) and 96.0 % with Halomonas desiderata FB2(T). DNA-DNA relatedness of strain SL014B-69(T) with strain SL014B-62A2, H. campisalis ATCC 700597(T) and H. desiderata DSM 9502(T) was 97.4, 42.9 and 36.8 %, respectively. On the basis of these data, a novel species of the genus Halomonas, Halomonas gudaonensis sp. nov., is proposed for strain SL014B-69(T) and SL014B-62A2. The type strain is SL014B-69(T) (=LMG 23610(T)=CGMCC 1.6133(T)).
从中国胜利油田沿海孤岛地区受原油污染的盐渍土中分离出两株中度嗜盐菌株SL014B - 69(T)和SL014B - 62A2；这些分离菌株为革兰氏阴性、杆状且具侧生鞭毛。在NaCl浓度为1 - 20%（w/v）、温度为10 - 42℃及pH值为8.0 - 9.0的条件下可生长。菌株SL014B - 69(T)以C(18 : 1)ω7c（28.61%）、C(19 : 1)环ω7c（27.97%）、C(16 : 0)（19.66%）和C(12 : 0) 3 - OH（8.87%）为主要脂肪酸，以Q9为主要泛醌，基因组DNA的G + C含量为64.0 mol%。基于16S rRNA基因序列的系统发育分析表明，这两株菌属于γ-变形菌纲中的嗜盐单胞菌属，与盐沼嗜盐单胞菌ATCC 700597(T)的16S rRNA基因序列相似性最高为96.4%，与需求嗜盐单胞菌FB2(T)的相似性为96.0%。菌株SL014B - 69(T)与菌株SL014B - 62A2、盐沼嗜盐单胞菌ATCC 700597(T)和需求嗜盐单胞菌DSM 9502(T)的DNA - DNA相关性分别为97.4%、42.9%和36.8%。基于这些数据，为菌株SL014B - 69(T)和SL014B - 62A2提出嗜盐单胞菌属的一个新种——孤岛嗜盐单胞菌（Halomonas gudaonensis sp. nov.）。模式菌株为SL014B - 69(T)（=LMG 23610(T)=CGMCC 1.6133(T)）。
