Derz Kerstin, Klinner Ulrich, Schuphan Ingolf, Stackebrandt Erko, Kroppenstedt Reiner M
Department of Ecology, Ecotoxicology and Ecochemistry, Aachen University, Worringerweg 1, D-52056 Aachen, Germany.
Unit of Applied Microbiology, Department of Microbiology and Genetics, Aachen University, Worringerweg 1, D-52056 Aachen, Germany.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2313-2317. doi: 10.1099/ijs.0.03003-0.
The taxonomic position of a polycyclic-aromatic-hydrocarbon-degrading bacterium, strain 17A3(T), isolated from contaminated soil was determined using a combination of phenotypic and genotypic properties. The isolate showed phenotypic properties that were diagnostic for species of the genus Mycobacterium. Comparative 16S rRNA gene sequence analysis assigned 17A3(T) to the 16S rRNA gene subgroup that contains Mycobacterium aurum, Mycobacterium austroafricanum, Mycobacterium vaccae and Mycobacterium vanbaalenii, but it could clearly be distinguished from these species using a combination of physiological, chemotaxonomic markers and internal rRNA gene spacer analyses. The data showed that strain 17A3(T) (=DSM 44605(T)=NRRL B-24244(T)) merits recognition as the type strain of a novel species of the genus Mycobacterium. The name Mycobacterium pyrenivorans sp. nov. is proposed for the species because of its ability to use pyrene as a sole source of carbon and energy.
利用表型和基因型特征相结合的方法，确定了从受污染土壤中分离出的一株多环芳烃降解细菌17A3(T)的分类地位。该分离株表现出的表型特征可作为分枝杆菌属物种的诊断依据。比较16S rRNA基因序列分析将17A3(T)归入包含金色分枝杆菌、非洲分枝杆菌、母牛分枝杆菌和范氏分枝杆菌的16S rRNA基因亚群，但通过生理、化学分类标记和内部rRNA基因间隔区分析相结合的方法，可将其与这些物种明确区分开来。数据表明，菌株17A3(T)(=DSM 44605(T)=NRRL B-24244(T))应被认定为分枝杆菌属一个新物种的模式菌株。由于该物种能够利用芘作为唯一碳源和能源，故将其命名为芘分枝杆菌新种（Mycobacterium pyrenivorans sp. nov.）。
