School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
Institute of Agri-resources and Regional Planning, CAAS, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2012 May;62(Pt 5):1204-1207. doi: 10.1099/ijs.0.033449-0. Epub 2011 Jul 8.
A gram-positive, acid-fast and rapidly growing rod, designated F4(T), was isolated from a soil sample of Haikou in China. The isolate shared 98.2 % 16S rRNA gene sequence similarity with Mycobacterium monacense B9-21-178(T), 96.2 % hsp65 sequence similarity with M. monacense FI-05352 and 79.6 % 16S-23S rRNA internal transcribed spacer sequence similarity with M. monacense B9-21-178(T). DNA-DNA relatedness between the isolate and M. monacense DSM 44395(T) was 43.5 %. The morphological analysis and physiological tests also showed that the isolate differed from any strain reported to date. The mycolic acid profile and the cellular fatty acid composition were also determined. On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, it was concluded that strain F4(T) ( = CGMCC 4.5724(T) = JCM 17423(T)) merited classification as the type strain of a novel species, for which the name Mycobacterium litorale sp. nov. is proposed.
一株革兰氏阳性、抗酸和快速生长的杆菌，命名为 F4(T)，从中国海口的土壤样本中分离得到。该分离株与 Mycobacterium monacense B9-21-178(T)的 16S rRNA 基因序列相似度为 98.2%，与 M. monacense FI-05352 的 hsp65 序列相似度为 96.2%，与 M. monacense B9-21-178(T)的 16S-23S rRNA 内部转录间隔区序列相似度为 79.6%。该分离株与 Mycobacterium monacense DSM 44395(T)的 DNA-DNA 同源性为 43.5%。形态分析和生理试验也表明，该分离株与迄今为止报道的任何菌株都不同。还测定了菌体内层酸的图谱和细胞脂肪酸组成。基于表型和化学分类学特征以及系统发育数据，结论是菌株 F4(T)（=CGMCC 4.5724(T)=JCM 17423(T)）值得分类为一个新种的模式菌株，提议将其命名为海洋分枝杆菌（Mycobacterium litorale）。
