Gupta P, Reddy G S N, Delille D, Shivaji S
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500 007, India.
Observatoire Oceanoloquie de Banyuls, Laboratoire Arago, Université Pierre et Marie Curie (Paris VI) CNRS, Institut National des Sciences de L'Univers BP 44, 66651 Banyuls sur Mer, France.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2375-2378. doi: 10.1099/ijs.0.63110-0.
Two coryneform bacteria were isolated from a penguin rookery soil sample collected in Antarctica, near the Indian station Dakshin Gangotri (strain Lz1y(T)), and from sea water from Kerguelen island, Antarctica (strain KGN15(T)). They have morphological and chemotaxonomic properties (peptidoglycan A4alpha type; major menaquinones MK-8, MK-9 and MK-10; predominant fatty acids anteiso-C(15 : 0) and anteiso-C(17 : 0)) that are characteristic of members of the genus Arthrobacter. The isolates shared 97.8 % 16S rRNA gene sequence similarity to each other and were most closely related to Arthrobacter sulfureus (about 98.5 % sequence similarity). DNA-DNA hybridization experiments revealed 50 % relatedness between the isolates, while the levels of DNA-DNA relatedness between strains Lz1y(T) and KGN15 (T) and their phylogenetic relative, A. sulfureus, were respectively 54 and 12 %. Based on the above data and distinct phenotypic differences between the isolates and A. sulfureus, two novel species are proposed, Arthrobacter gangotriensis sp. nov. (type strain Lz1y(T)=DSM 15796(T)=JCM 12166(T)) and Arthrobacter kerguelensis sp. nov. (type strain KGN15(T)=DSM 15797(T)=JCM 12165(T)).
从南极靠近印度达克辛甘戈特里站采集的企鹅栖息地土壤样本（菌株Lz1y(T)）以及南极凯尔盖朗岛的海水中（菌株KGN15(T)）分离出了两株棒状杆菌。它们具有节杆菌属成员所特有的形态和化学分类学特性（肽聚糖为A4α型；主要甲基萘醌为MK - 8、MK - 9和MK - 10；主要脂肪酸为anteiso - C(15 : 0)和anteiso - C(17 : 0)）。这些分离株彼此之间16S rRNA基因序列相似性为97.8%，与硫节杆菌最为接近（序列相似性约为98.5%）。DNA - DNA杂交实验表明这些分离株之间的相关性为50%，而菌株Lz1y(T)和KGN15(T)与其系统发育相关的硫节杆菌之间的DNA - DNA相关性水平分别为54%和12%。基于上述数据以及分离株与硫节杆菌之间明显的表型差异，提出了两个新物种，即冈戈特里节杆菌（Arthrobacter gangotriensis sp. nov.）（模式菌株Lz1y(T)=DSM 15796(T)=JCM 12166(T)）和凯尔盖朗节杆菌（Arthrobacter kerguelensis sp. nov.）（模式菌株KGN15(T)=DSM 15797(T)=JCM 12165(T)）。
