Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2263-2266. doi: 10.1099/ijs.0.012989-0. Epub 2009 Sep 25.
A bacterial strain, SPC26(T), was isolated from a sediment sample of the Southern Ocean off Antarctica. The strain was Gram-staining- and catalase-positive and contained lysine and alanine in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C₁₅:₀ (54.92 %), iso-C₁₅:₀ (11.47 %), anteiso-C₁₇:₀ (6.48 %) and anteiso-C₁₅:₁ (6.38 %) and the major menaquinones were MK-8, MK-9 and MK-10. The major polar lipids were phosphatidylethanolamine and diphosphatidylglycerol. The G+C content was 68 ± 0.5 mol%. Based on 16S rRNA gene sequence similarities, the nearest phylogenetic neighbours of strain SPC26(T) were identified as Arthrobacter gangotriensis Lz1y(T) (98.8 %), A. sulfureus DSM 20167(T) (98.6 %), A. psychrophenolicus DSM 15454(T) (97.9 %) and A. kerguelensis KGN15(T) (97.5). With these strains, strain SPC26(T) exhibited DNA-DNA relatedness values of 36, 21, 12 and 10 %, respectively. Therefore, on the basis of 16S rRNA gene sequence comparisons, phylogenetic analysis, phenotypic characteristics and DNA-DNA relatedness, it is proposed that strain SPC26(T) represents a novel species of Arthrobacter, for which the name Arthrobacter antarcticus sp. nov. is proposed, with strain SPC26(T) (=LMG 24542(T) =NCCB 100228(T)) as the type strain.
一株细菌菌株 SPC26(T) 从南极洲南大洋的沉积物样本中分离得到。该菌株革兰氏染色阳性，过氧化氢酶阳性，细胞壁肽聚糖中含有赖氨酸和丙氨酸。主要细胞脂肪酸为anteiso-C₁₅:₀（54.92%）、iso-C₁₅:₀（11.47%）、anteiso-C₁₇:₀（6.48%）和anteiso-C₁₅:₁（6.38%），主要的menaquinones 为 MK-8、MK-9 和 MK-10。主要的极性脂为磷脂酰乙醇胺和双磷脂酰甘油。G+C 含量为 68 ± 0.5 mol%。基于 16S rRNA 基因序列相似性，菌株 SPC26(T) 的最接近的系统发育近邻鉴定为 Gangotri 节杆菌 Lz1y(T)（98.8%）、黄杆菌 DSM 20167(T)（98.6%）、嗜冷节杆菌 DSM 15454(T)（97.9%）和 Kerguelensis KGN15(T)（97.5%）。与这些菌株相比，菌株 SPC26(T) 的 DNA-DNA 同源性值分别为 36%、21%、12%和 10%。因此，基于 16S rRNA 基因序列比较、系统发育分析、表型特征和 DNA-DNA 同源性，建议菌株 SPC26(T) 代表一个新的南极节杆菌种，命名为南极节杆菌新种，菌株 SPC26(T)=LMG 24542(T)=NCCB 100228(T)为模式菌株。
