Mitusch Rolf, Luedemann Jan, Wood William G, Berger Klaus, Schminke Ulf, Suter Matthias, Kessler Christof, John Ulrich, Rupp Jan, Kentsch Michael, Maass Matthias
Medical Department, Hanse-Hospital Stralsund, Teaching Hospital of the Ernst-Moritz-Arndt-University of Greifswald, Post Box 2341, D-18410 Stralsund, Germany.
Arterioscler Thromb Vasc Biol. 2005 Feb;25(2):386-91. doi: 10.1161/01.ATV.0000151284.49967.a7. Epub 2004 Nov 18.
Chlamydia pneumoniae infection has been associated with atherosclerosis, but serodiagnosis is unreliable in predicting vascular infection. Direct detection of circulating chlamydial DNA in peripheral blood mononuclear cells (PBMCs) was thus evaluated as a marker for cardiovascular risk in a general population survey using the common carotid intima-media thickness (IMT) as surrogate marker of asymptomatic atherosclerosis.
C pneumoniae DNA in PBMCs was determined by nested polymerase chain reaction and associated with IMT for 1032 healthy participants of a general population survey who were within the highest or lowest IMT distribution quartile. C pneumoniae DNA was more prevalent in those with increased IMT (13.4% versus 10.7%), but this was not significant in univariate and of borderline significance in multivariate analysis. Testing for potential effect modifications by known strong determinants of an increased IMT in group interaction analysis revealed an independent association between C pneumoniae DNA and IMT in normotensive subjects (odds ratio [OR], 2.06; 95% CI, 1.05 to 4.03; P=0.04) and in those <70 years old (OR, 1.84; 95% CI, 1.06 to 3.19; P=0.03).
Asymptomatic atherosclerosis is associated with circulating C pneumoniae DNA independently of classical cardiovascular risk factors in normotensive subjects and those <70 years old. C pneumoniae has been implicated in atherogenesis. We determined the association of chlamydial DNA in peripheral blood mononuclear cells with the carotid intima-media thickness from 1032 healthy subjects from a general population survey. A stratified group interaction analysis revealed an independent association in normotensive subjects and those <70 years old.
肺炎衣原体感染与动脉粥样硬化有关，但血清学诊断在预测血管感染方面并不可靠。因此，在一项普通人群调查中，以外周血单核细胞（PBMC）中循环衣原体DNA的直接检测作为心血管风险标志物，使用颈总动脉内膜中层厚度（IMT）作为无症状动脉粥样硬化的替代标志物。
通过巢式聚合酶链反应测定PBMC中的肺炎衣原体DNA，并将其与1032名普通人群调查中的健康参与者的IMT相关联，这些参与者处于IMT分布的最高或最低四分位数。IMT增加的人群中肺炎衣原体DNA更为普遍（13.4%对10.7%），但在单变量分析中不显著，在多变量分析中具有临界显著性。在组间交互分析中，通过已知的IMT增加的强决定因素测试潜在的效应修饰，结果显示在血压正常的受试者（优势比[OR]，2.06；95%可信区间，1.05至4.03；P=0.04）和年龄<70岁的受试者（OR，1.84；95%可信区间，1.06至3.19；P=0.03）中，肺炎衣原体DNA与IMT之间存在独立关联。
在血压正常的受试者和年龄<70岁的受试者中，无症状动脉粥样硬化与循环肺炎衣原体DNA相关，且独立于经典心血管危险因素。肺炎衣原体与动脉粥样硬化的发生有关。我们在一项普通人群调查中，确定了1032名健康受试者外周血单核细胞中的衣原体DNA与颈动脉内膜中层厚度之间的关联。分层组间交互分析显示在血压正常的受试者和年龄<70岁的受试者中存在独立关联。
