Liu Jin-Hua, Okazaki Katsunori, Mweene Aaron, Shi Wei-Min, Wu Qing-Min, Su Jing-Liang, Zhang Guo-Zhong, Bai Gui-Rong, Kida Hiroshi
Department of Infectious Disease, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
Virus Genes. 2004 Dec;29(3):329-34. doi: 10.1007/s11262-004-7436-x.
The hemagglutinin (HA) genes of 12 H9N2 influenza virus strains isolated from chickens in Mainland China during the period 1995-2002 were genetically analyzed. All the isolates possessed the same amino acid motif -R-S-S-R/G-L- at the cleavage site of HA. Except for the conserved amino acids, as is the case in the other avian influenza viruses, located in the receptor binding site, all of the 12 isolates possessed N at amino acid position 183; A, T, or V at position 190; K at position 137, whereas the representative strains of the other lineage (except Dk/HK/Y280/97-like lineage) virus of H9N2 viruses had H, E, and R at these positions respectively. These could be considered as the partial molecular markers of the H9 viruses isolated from chickens in Mainland China. Phylogenetic analyses showed HA genes of these isolates belonged to that of A/duck/Hong Kong/Y280/97-like virus lineage. No A/quail/Hong Kong/Gl/97-like virus was found in chicken, population since the outbreak of H9N2 influenza in Mainland China in 1992. The available evidence indicates that HA genes of H9 influenza virus circulating in Mainland China during the past years were well conserved.
对1995年至2002年期间从中国大陆鸡群中分离出的12株H9N2流感病毒株的血凝素（HA）基因进行了遗传分析。所有分离株在HA裂解位点均具有相同的氨基酸基序-R-S-S-R/G-L-。除了位于受体结合位点的保守氨基酸外，其他禽流感病毒也存在这种情况，这12株分离株在氨基酸位置183处均为N；在位置190处为A、T或V；在位置137处为K，而H9N2病毒其他谱系（除Dk/HK/Y280/97样谱系外）的代表性毒株在这些位置分别为H、E和R。这些可被视为从中国大陆鸡群中分离出的H9病毒的部分分子标记。系统发育分析表明，这些分离株的HA基因属于A/duck/Hong Kong/Y280/97样病毒谱系。自1992年中国大陆爆发H9N2流感以来，在鸡群中未发现A/quail/Hong Kong/Gl/97样病毒。现有证据表明，过去几年在中国大陆流行的H9流感病毒的HA基因高度保守。
