蓝蓟深蓝色蜂花粉花青素成分的液相色谱-质谱分析

Liquid chromatographic-mass spectrometric analysis of anthocyanin composition of dark blue bee pollen from Echium plantagineum.

作者信息

Di Paola-Naranjo Romina Daniela, Sánchez-Sánchez José, González-Paramás Ana María, Rivas-Gonzalo Julián Carlos

机构信息

Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca 37007, Spain.

出版信息

J Chromatogr A. 2004 Oct 29;1054(1-2):205-10. doi: 10.1016/j.chroma.2004.05.023.

DOI:10.1016/j.chroma.2004.05.023

PMID:15553145

Abstract

Dark blue bee pollen samples from pollinic type Echium plantegineum were analysed in order to identify and quantify their anthocyanin pigments. Five samples were collected from different apicultural Spanish regions and the anthocyanin composition was determined by HPLC with diode array and MS detection. Eight different pigments were identified, the principal anthocyanin being petunidin-3-O-rutinoside. The other pigments found were delphinidin, cyanidin and petunidin-3-O-glucoside; delphinidin, cyanidin, peonidin and malvidin-3-O-rutinoside and cyanidin-3-(6"-malonylglucoside). The anthocyanin content ranged from 45 to 80 mg/100 g of blue pollen, which could represent a significant source of phytochemicals. Minor variations in the anthocyanin profiles were found, which could be explained by the geographical differences between collection regions.

摘要

对来自花粉类型为蓝蓟（Echium plantegineum）的深蓝色蜂花粉样本进行分析，以鉴定和定量其中的花青素色素。从西班牙不同养蜂地区采集了五个样本，并通过配备二极管阵列和质谱检测的高效液相色谱法测定花青素组成。鉴定出八种不同的色素，主要花青素为矮牵牛素 - 3 - O - 芸香糖苷。发现的其他色素有飞燕草素、矢车菊素和矮牵牛素 - 3 - O - 葡萄糖苷；飞燕草素、矢车菊素、芍药素和锦葵素 - 3 - O - 芸香糖苷以及矢车菊素 - 3 - (6'' - 丙二酰葡萄糖苷)。花青素含量在每100克蓝色花粉中为45至80毫克，这可能是植物化学物质的重要来源。发现花青素谱存在微小差异，这可以用采集地区之间的地理差异来解释。