Zhang Yue-hong, Gao Lan-xing, Jin Hong, Xu Zhi-qin, Nan Wen-kao, Wang Xian-yuan
Institue of Health and Environmental Medicine, Tianjin 300050, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2003 May;19(2):182-4.
To study the effects of genistein on proliferation and differentiation of osteoblasts in neonatal rat calvaria cultures.
Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the presence of different doses of genistein (10(-5) mol/L, 10(-6) mol/L and 10(-7) mol/L). The proliferation and DNA and collagen synthesis of osteoblasts were assayed by MTT method and 3H-TdR and 3H-proline incorporation. The activity of ALP were measured by ALP assay kit.
Genistein significantly increased osteoblast 3H-TdR and 3H-proline incorporation and MTT, 10(-6) mol/L genistein increased ALP activity.
Genistein increased osteoblast DNA and collagen synthesis in neonatal rat calvaria cultures, and promoted osteoblast proliferation and differentiation.
研究金雀异黄素对新生大鼠颅骨培养成骨细胞增殖和分化的影响。
通过胰蛋白酶和胶原酶消化从新生大鼠颅骨分离成骨细胞，并在不同剂量的金雀异黄素（10⁻⁵mol/L、10⁻⁶mol/L和10⁻⁷mol/L）存在下培养。采用MTT法、³H-TdR掺入法和³H-脯氨酸掺入法检测成骨细胞的增殖以及DNA和胶原蛋白合成。用碱性磷酸酶检测试剂盒测量碱性磷酸酶的活性。
金雀异黄素显著增加成骨细胞³H-TdR和³H-脯氨酸掺入以及MTT值，10⁻⁶mol/L金雀异黄素增加碱性磷酸酶活性。
金雀异黄素增加新生大鼠颅骨培养中成骨细胞的DNA和胶原蛋白合成，并促进成骨细胞增殖和分化。
