Li Bin-bin, Yu Shi-feng, Pang Shu-zhen
Department of Oral Pathology, Peking University School of Stomatology, Beijing 100081, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2005 May;40(3):237-40.
To study the effect of genistein on the proliferation, differentiation and apoptosis of the osteoblasts in vitro.
The primary osteoblasts (OBs) were obtained from the rat calvaria and the cell line of osteosarcoma-UMR-106 served as control. The cells in the experiment group were grown in 10% (volume fraction) fetal calf serum (FCS) + alphaMEM + various concentrations of genistein. The control groups were grown in 10% FCS + alphaMEM. The growth of OBs was assessed by flow cytometry and MTT method. The differentiation of OBs was examined by the alkaline phosphatase (ALP) activity.
Flow cytometry analysis and MTT showed that genistein could prompt primary OB from stage G(0) (G(1)) to stage S, G(2) or M. By contrast, genistein had no effect on the cell cycle of UMR-106, but could induce its apoptosis. Additionally, the results of ALP activity showed that genistein stimulated the differentiation of primary OB.
Genistein can stimulate the proliferation and differentiation of the primary osteoblasts in some degree, and induce the apoptosis of osteosarcoma cells.
研究染料木黄酮对体外培养的成骨细胞增殖、分化及凋亡的影响。
取大鼠颅骨获得原代成骨细胞，以骨肉瘤细胞系UMR-106作为对照。实验组细胞在含10%（体积分数）胎牛血清（FCS）的α-MEM培养基中加入不同浓度的染料木黄酮进行培养。对照组细胞在含10% FCS的α-MEM培养基中培养。采用流式细胞术和MTT法评估成骨细胞的生长情况。通过碱性磷酸酶（ALP）活性检测成骨细胞的分化情况。
流式细胞术分析和MTT结果显示，染料木黄酮可促使原代成骨细胞从G(0)（G(1)）期进入S期、G(2)期或M期。相比之下，染料木黄酮对UMR-106细胞周期无影响，但可诱导其凋亡。此外，ALP活性结果表明，染料木黄酮可促进原代成骨细胞的分化。
染料木黄酮可在一定程度上促进原代成骨细胞的增殖和分化，并诱导骨肉瘤细胞凋亡。
